Chlamydophila psittaci (Lillie) Everett et al. (ATCC® VR-351)

Classification: Chlamydiaceae  /  Product Format: frozen

Classification Chlamydiaceae
Deposited As Ornithosis
Agent Chlamydophila psittaci (Lillie) Everett et al.
Strain Ornithosis strain Texas Turkey [strain Texas turkey]
Applications
Addition of cycloheximide recommended for passage in cell culture.
Biosafety Level 3

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
turkey spleen, Texas
Product Format frozen
Storage Conditions -70°C or colder
Comments
Centrifugation (2000 X g for 1 hr.) and addition of cycloheximide recommended for passage in cell culture. *Requests for this agent must carry signed statement assuming all risks and responsibility for lab handling.
Triturate fluid several times to disperse chlamydia. For cell culture, centrifuge inoculumn, adsorb for 1 hour and feed with HEPES buffered culture medium.
Effect on Host
Cytopathic effects (inclusions) in tissue culture
Death of embryo
Recommended Host
McCoy cells (ATCC CRL-1696)
chicken embryo, yolk sac; mouse; turkey; McCoy cells (ATCC CRL-1696)
Growth Conditions
Incubation: 5 days (approximately)
Effect on Host
Cytopathic effects (inclusions) in tissue culture
Death of embryo
Name of Depositor J Schachter
Source
turkey spleen, Texas
References

Meyer KF, Eddie B. Characteristics of a psittacosis viral agent isolated from a turkey. Proc. Soc. Exp. Biol. Med. 83: 99-101, 1953. PubMed: 13064188