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Monoclonal antibody against FOS peptide (359-378) [LA 054]

VR-1518AF-MAB ™

Immunization Schedule: Approximately 70 g of coupled peptide in 300 l of a 1:1 mixture of phosphate buffered saline pH 7.4 and complete Freund's adjuvant administered intraperitoneally on day 0; followed by an equal amount of conjugate in 300 l of a 1:1 mixture of phosphate buffered saline pH 7.4 and 10 mg/mL alum administered intraperitoneally on day 14; followed by 70 g of coupled peptide in 200 l of phosphate buffered saline pH 7.4 administered intravenously on day 51. The spleen was removed on day 56.
Product category
Viruses
Product type
Antibody
Antibody class
IgG1
Source
Male 129GIX+ mice were used for immunization.
Immunogen species
Synthetic peptide RKGSSSNEPSSDSLSSPTLL(C) (residues 359-378 of the FOS sequence) coupled to keyhole limpet hemocyanin
Product format
Freeze-dried
Storage conditions
2°C to 8°C
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Mission Collection Item
This is a Mission Collection Item.
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General

Specific applications
Source and Sex of Animals: Male 129GIX+ mice were used for immunization.
Cross-reactivity: In immunoblot screening a protein of approximately 55 kd is detected in a fibroblast cell line transformed by fes, sis, and scr.
Approximately 1:100,000 against the unconjugated synthetic peptide in an indirect enzyme-linked immunosorbent assay
Animal
Male 129GIX+ mice were used for immunization.
Adjuvant
complete Freund's adjuvant
Material development
Source and Sex of Animals: Male 129GIX+ mice were used for immunization.
Fusion Partners: 1.3 x 10(8) nucleated spleen cells with 2 x 10(7) SP2/0 non-secreting myeloma cells
Immunization Schedule: Approximately 70 mg of coupled peptide in 300 ml of a 1:1 mixture of phosphate buffered saline pH 7.4 and complete Freund's adjuvant administered intraperitoneally on day 0;
followed by an equal amount of conjugate in 300 ml of a 1:1 mixture of phosphate buffered saline pH 7.4 and 10 mg/ml alum administered intraperitoneally on day 14;
followed by 70 mg of coupled peptide in 200 ml of phosphate buffered saline pH 7.4 administered intravenously on day 51. The spleen was removed on day 56.
Cross reactivity
In immunoblot screening a protein of approximately 55 kd is detected in a fibroblast cell line transformed by fes, sis, and scr.

Characteristics

Comments
Preparation: Monoclonal antibody is in ascites fluid collected from 2-06-87 to 7-06-87.
The ascites fluid was ammonium sulfate precipitated, reconstituted, filter sterilized, aliquoted 1 ml per vial, and lyophilized. The sterility is not guaranteed. Resuspend in 1 ml distilled water.
Approximately 1:100,000 against the unconjugated synthetic peptide in an indirect enzyme-linked immunosorbent assay
Vials may be stored at 2-8°C.

Quality control specifications

Mycoplasma contamination
Not detected

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

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Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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