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Chlamydia trachomatis

VR-1477

Chlamydia trachomatis strain TW-3 [Trachoma type C strain TW-3] is propagated in HEp-2 cells (ATCC CCL-23). This strain was isolated in Taiwan from a specimen taken from the human conjunctiva. Use this bacterium in your sexually transmitted disease research.
Product category
Bacteria
Classification
Chlamydiaceae, Chlamydia
Strain designation
TW-3 [Trachoma type C strain TW-3]
Type strain
No
Isolation source
Specimen from human conjunctiva.
Geographical isolation
Taiwan
Applications
Infectious disease research
Sexually transmitted disease research
Product format
Frozen
Storage conditions
-70°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information

General

Specific applications
Sexually Transmitted Disease Research

Characteristics

Serotype
C
Comments
FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.
This strain is a tissue culture-adapted version of strain TW-3 (ATCC VR-578) which is grown in embryonated chicken eggs.
Before infecting host cells, add 1-mm glass beads and vortex stock material for 45 seconds. Aspirate fluid. Infect shell vial monolayer with 0.2 mL of material.
Centrifuge at 800 x G for 1 hour. Feed with 1.0 mL EMEM (HEPES buffered), 10% FBS (screened for chlamydia antibodies).
Serotype C
Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC CRL-1696) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus.
Mycoplasma contamination
Detected

Handling information

Host
Effects
No CPE; requires IFA for visualization of inclusion bodies
Complete medium
DMEM (ATCC 30-2002) + 10% prescreened FBS + 10 mM HEPES + 2 µg/mL Cycloheximide (Sigma C-4859 Ready-Made)
Temperature
37°C
Atmosphere
95% Air, 5% CO2
Incubation
48 to 72 hours, a 5% CO2 in air atmosphere is recommended.
Recommendation for infection
Add glass beads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for 1 hour. Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1-2mg/mL cycloheximide. Incubate at 37°C for 48 to 72 hours, a 5% CO2 in air atmosphere is recommended. Incubation: 48 to 72 hours, a 5% CO2 in air atmosphere is recommended.
Handling notes

FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.

ATCC VR-1477 is a TC-adapted version of the TW-3 strain that was previously grown in embryonated chicken eggs (ATCC VR-578).  Biosafety level 3 facilities and practices are indicated for activities with high potential for droplet or aerosol production, CDC BMBL 4th edition.

Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC CRL-1696) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus.

Key abbreviations
°C, Degrees Celsius
CO2, Carbon dioxide
DMEM, Dulbecco's Modified Eagle's Medium
FA, Fluorescent antibody assay
FBS, Fetal bovine serum
g, Acceleration of gravity
HEPES, N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)

Quality control specifications

Volume
1.0 mL

History

Deposited as
Chlamydia trachomatis (Busacca) Rake
Depositors
Kuo
Type of isolate
Human

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Grayston JT, et al. Trachoma-studies of etiology, laboratory diagnosis, and prevention. JAMA 172: 1577-1586, 1960. PubMed: 13829148

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