pHEBo1 [pHEBo] (ATCC® 39820)

Applications: shuttle vector  /  Depositors: Wisconsin Alumni Res. Fndn., HW Bremer, Wisconsin Alumni Res. Fndn.

Designations pHEBo1 [pHEBo]
Permits and Restrictions

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Depositors Wisconsin Alumni Res. Fndn., HW Bremer, Wisconsin Alumni Res. Fndn.
U.S. Patent
Disclosure FPAT
Biosafety Level 1
Distribution host: Escherichia coli
U.S. Patent
Vector Information
Size (kb): 6.9000000953674320
Vector: pHEBo1 (plasmid)
Promoters: Promoter HSV TK
Construction: EBV, HSV-1, pBR322
Construct size (kb): 6.900000095367432
Features: marker(s): ampR, hygR
promoter: HSV TK
replicon: pMB1, EBV oriP
shuttle vector
Restriction digests of the clone give the following sizes (kb): BamHI--6.9; ClaI--6.9; EcoRI--4.4, 2.3; EcoRV--4.6, 2.4; HindIII--6.9; SalI--6.9.
A map is provided in this reference.
Shuttle vector that can replicate and be selected in Escherichia coli and mammalian cells.
The order of the major features in this plasmid is: EcoRI - ClaI - HindIII - BamHI - SalI - oriP - HSV-1 tk polyadenylation - <- hygR - HSV-1 tk promoter - < - ampR.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Sugden WM. Recombinant vector and eukaryotic host transformed thereby. US Patent 4,686,186 dated Aug 11 1987

. Molecular cloning: a laboratory manual. Cold Spring Harbor, NY: CSHL Press; 1989.

Shipped freeze-dried