pHEBo1 [pHEBo] (ATCC® 39820)

Applications: shuttle vector  /  Depositors: Wisconsin Alumni Res. Fndn., HW Bremer, Wisconsin Alumni Res. Fndn.

Designations pHEBo1 [pHEBo]
Permits and Restrictions

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Depositors Wisconsin Alumni Res. Fndn., HW Bremer, Wisconsin Alumni Res. Fndn.
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level 1
Distribution host: Escherichia coli
Vector Information
Size (kb): 6.9000000953674320
Vector: pHEBo1 (plasmid)
Promoters: Promoter HSV TK
Construction: EBV, HSV-1, pBR322
Construct size (kb): 6.900000095367432
Features: marker(s): ampR, hygR
promoter: HSV TK
replicon: pMB1, EBV oriP
shuttle vector
Restriction digests of the clone give the following sizes (kb): BamHI--6.9; ClaI--6.9; EcoRI--4.4, 2.3; EcoRV--4.6, 2.4; HindIII--6.9; SalI--6.9.
A map is provided in this reference.
Shuttle vector that can replicate and be selected in Escherichia coli and mammalian cells.
The order of the major features in this plasmid is: EcoRI - ClaI - HindIII - BamHI - SalI - oriP - HSV-1 tk polyadenylation - <- hygR - HSV-1 tk promoter - < - ampR.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Sugden WM. Recombinant vector and eukaryotic host transformed thereby. US Patent 4,686,186 dated Aug 11 1987

. Molecular cloning: a laboratory manual. Cold Spring Harbor, NY: CSHL Press; 1989.

Shipped freeze-dried