pBR322 (ATCC® 31344)

Depositors: Genentech, Inc.

Permits and Restrictions

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Designations pBR322
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Depositors Genentech, Inc.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli RR1; K-12 RR1
Vector Information
Size (kb): 4.363
Vector: pBR322 (plasmid)
Type: cloning
Construction:
Marker(s):ampR,tetR
Construct size (kb): 4.363
Features: marker(s): ampR
marker(s): tetR
replicon: pMB1
Comments
General purpose plasmid vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Bolivar F, et al. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2: 95-113, 1977. PubMed: 344137

Riggs AD. Method for microbial polypeptide expression. US Patent 4,366,246 dated Dec 28 1982

Goeddel DV, Heyneker HL. Method of constructing a replicable cloning vehicle having quasi-synthetic genes. US Patent 4,342,832 dated Aug 3 1982

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 31344