pBR322 (ATCC® 31344)

Depositors: Genentech, Inc.

Designations pBR322
Depositors Genentech, Inc.
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level 1
Distribution host: Escherichia coli RR1; K-12 RR1
Vector Information
Size (kb): 4.363
Vector: pBR322 (plasmid)
Type: cloning
Construct size (kb): 4.363
Features: marker(s): ampR
marker(s): tetR
replicon: pMB1
General purpose plasmid vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Bolivar F, et al. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2: 95-113, 1977. PubMed: 344137

Riggs AD. Method for microbial polypeptide expression. US Patent 4,366,246 dated Dec 28 1982

Goeddel DV, Heyneker HL. Method of constructing a replicable cloning vehicle having quasi-synthetic genes. US Patent 4,342,832 dated Aug 3 1982

Shipped freeze-dried
Shipping Information Distributed: freeze-dried