Related Products

  • S. cerevisiae/E. coli marker swap vectors (ATCC® 87561)

    ATCC® Number: 87561

    Depositor: FR Cross, DJ Stillman

    For-Profit:   $1,500.00 Non-Profit:   $1,500.00

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    Applications: This is a set of 15 marker swap vectors, each containing a gene disruption conversion cassette for conversion between standard markers used for transformation selection in Saccharomyces cerevisiae.  The vectors are useful in changing markers for gene disruptions or for changing markers on plasmids.

    To convert the host phenotype from the existing yeast auxotrophic marker to a new marker (eg. HIS3 → LEU2), transform with the restriction enzyme digested vector (eg. ApaI+PstI digested pHL3) and select for the appropriate phenotype (for pHL3, Leu+).

    Some combinations of marker swap plasmids and target locus may result in relatively high reversion rates.  In most, but not all cases, the frequencies of successful convertants are greater than 30%.  When swapping markers on an episomal plasmid, appropriate phenotype may result from loss of the plasmid unless a second selectable or scorable marker is used to ensure plasmid maintenance.
  • Molecular Grade Water (ATCC® 60-2450)

    ATCC® Number: 60-2450

    Quantity: 1 liter
    Storage: Store at 15°C to 30°C
    Applications:
    For use in all molecular biology procedures that require water, including PCR. Distilled, deionized, sterile-filtered.

    For-Profit:   $37.00 Non-Profit:   $37.00

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  • pJD Plasmid Control Series for Mutation Analysis (ATCC® 87584)

    ATCC® Number: 87584

    Applications:
    A set of plasmids to be used as controls for detecting mutations or validating methods of mutation scanning.
    These plasmids are designed so that the same PCR primer pair can be used on each plasmid.

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    Product Format: freeze-dried
    Comments:
    Primers have been tested that yield DNA fragments sizes ranging between 203 and 1455 bp.
    A set of plasmids to be used as controls for detecting mutations or validating methods of mutation scanning. The mutations are either point variants (A, T, C or G at nt 272) or insertions of one to five nucleotides (starting at nt 274).
    The plasmids were derived from pUC19 (GenBank M77789), with each plasmid containing a 10 to 15 base pair replacement between the BamHI and EcoRI sites. These plasmids are designed so that the same PCR primer pair can be used on each plasmid.
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