Aspergillus brasiliensis Varga et al.

Certified Reference Material produced under an ISO 17034 accredited process.

This strain was recently re-named as Aspergillus brasiliensis according to the reference Varga et al. 2007. 

Use of impedance for preservative efficacy testing

1. Open vial according to enclosed instructions.
2. From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet to rehydrate it.  Stir to form a suspension.
3. Aseptically transfer the rehydrated pellet (suspension) back into the test tube with the sterile distilled water.  Mix well.
4. Let the test tube sit at room temperature (~23 °C) for at least 2 hours, if not overnight.
5. Mix the suspension well.  Use several drops to inoculate a test tube or slant or a plate with recommended medium.
6. Incubate the test tube or plate at the temperature recommended for 2-11 days.

Sporulation may be inhibited in plates sealed completely with tape.  Colonies grown directly from rehydrated spores may exhibit sectoring, with areas of varying levels of sporulation.  Intermittent light exposure helps sporulation after incubating 48 hours at 30°C.

This strain was identified as belonging to the new species Aspergillus brasiliensis, described in the following two publications:

1. Varga et al. 2007. Int. J. Syst. Evol. Microbiol. 57:1925-1932.
2. Houseknecht, J., Stamenova, E., Suh, S.-O., Beck, B., McKee, M. & Zhou. J. 2008. Reclassification of ATCC^® 16404™ and ATCC^® 9642™ as Aspergillus brasiliensis. Pharmaceutical Microbiology Forum Newsletter 14: 2-8.

Certified Reference Material produced under an ISO 17034 accredited process.

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