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c9115 [9115]

70874

Organism
Saccharomyces cerevisiae Meyen ex E.C. Hansen
Classification
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
Clone type
Clone
Applications
Molecular biology
Product format
Frozen
Mission Collection Item
This is a Mission Collection Item.

Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
The ATCC encourages users of this material to communicate their results to us.

Characteristics

Comments
Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 1.251, 0.935, 1.917, 1.821, 1.805, 3.357, 0.812, 0.435, 1.845, 3.368, 5.251, 5.171, 0.997, 0.952, 0.637, 0.469, 2.283, 1.448, 1.004, 0.934.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Overlaps clones with ATCC number(s): 70132, 70151, 70363, 70459, 70469.
NOTE: Because of the large number of simultaneous deposits from Dr. Olson, the ATCC has not verified that the characteristics of the material supplied here are as published. The ATCC encourages users of this material to communicate their results to us.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
0.0
Intact vector size
6.430
Vector name
pHC79
Type of vector
cosmid
Construction
pBR322, Charon 4A
Host range
Escherichia coli
Vector end
BamHI
Vector information
Cross references: DNA Seq. Acc.: L08873
Cloning sites
EcoRI; ClaI; BamHI; SalI; EcaI; PstI
Markers
ampR; tetR
Replicon
pMB1
Terminator
none

Insert information

Type of DNA
genomic
Insert strain
AB972 (ATCC 76269)
Insert information
DESCRIPTION OF INSERT COMPONENT:
Cross references: DNA Seq. Acc.: U18916
Nucleotides 1-13368 of the insert correspond to
nucleotides 54936-68303 of U18916.
DNA Seq. Acc.: U18917
Nucleotides ? of the insert correspond to
nucleotides 1-23020 of U18917.
Genome
Saccharomyces cerevisiae
Chromosome
V
Gene name
protein phosphatase type I; GDP dissociation inhibitor; 3-methyladenine DNA glycosylase; ubiquitin-specific protease
Gene product
protein phosphatase, type 1(protein phosphatase type I) [GDI1]
Gene symbol
GLC7; GDI1; MAG1; UBP5
Contains complete coding sequence
Unknown
Insert end
MboI

Handling information

Host
Escherichia coli HB101 (ATCC 33694)
Medium
Temperature
30°C
Handling notes
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we
recommend streaking on LB + amp plates at 30C and picking small colonies for
liquid culture.
- ATCC staff

Overlaps clones with ATCC number(s): 70132, 70151, 70363, 70459, 70469.
- personal communication

Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered
as in the genome: 1.251, 0.935, 1.917, 1.821, 1.805, 3.357, 0.812, 0.435, 1.845,
3.368, 5.251, 5.171, 0.997, 0.952, 0.637, 0.469, 2.283, 1.448, 1.004, 0.934.
- Genetics 134: 81-150, 1993


Because of the large number of simultaneous deposits from Dr. Olson's
laboratory, the ATCC has not verified that the characteristics of the material
supplied here are as published. The ATCC encourages users of this material to
communicate results of their tests to us.

History

Depositors
MV Olson, L Riles
Cross references
GenBank U18916
GenBank U18917

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151

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