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GenBank Definition
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Saccharomyces cerevisiae putative protein kinase (MRK1) gene,
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Species
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Saccharomyces cerevisiae Meyen ex E.C. Hansen
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Vector
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Construct size (kb): 0.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdaMG3
Intact vector size:
Type of vector: phage
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI
Polylinker sites:
Construction: L47.1
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
promoter: none
replicon: lambda
terminator: none
Cross references:
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Insert
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DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Saccharomyces cerevisiae Gene symbol: MRK1 Gene name: glycogen synthase kinase-3-related Contains complete coding sequence?: U Chromosome: IV Strain: AB972 ( ATCC 76269) Type of DNA: genomic Insert end: Insert end: Insert size (kb): Cross references: Gene product: glycogen synthase kinase-3-related [MRK1]
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Biosafety Level
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1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
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Shipping Information
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Distributed: freeze-dried bacteria-free lysate
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Comments
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Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 1.798, 0.826, 2.116, 1.826, 2.335, 1.163, 0.704, 2.084, 0.899, 0.779, 0.905, 0.773, 0.450, 0.586, 1.134. Confirmed to contain EcoRI/HindIII fragments of the following sizes (kb): 0.45, 0.59, 0.64, 0.70, 0.77, 0.78, 0.82, 0.88, 0.90, 0.90, 1.1, 1.2, 1.4, 1.8, 1.8. MRK1 occurs in a 3.9 kb EcoRI fragment, and in a 1.8 kb EcoRI/SpeI subfragment. Overlaps clones with ATCC number(s): 70751, 70996.
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Classification
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Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
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References
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Olson MV, et al. Random-clone strategy for genomic restriction mapping in yeast. Proc. Natl. Acad. Sci. USA 83: 7826-7830, 1986. PubMed: 3463999
Hardy TA, et al. Novel Saccharomyces cerevisiae gene, MRK1, encoding a putative protein kinase with similarity to mammalian glycogen synthase kinase-3 and Drosophila zeste-white/shaggy. Biochem. Biophys. Res. Commun. 208: 728-734, 1995. PubMed: 7695629
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