7C2C5C12 (ATCC® HB-8678)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain BALB/c
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Trichinella spiralis
Cellular Products
immunoglobulin; monoclonal antibody; against Trichinella spiralis
Tumorigenic Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Complete Growth Medium Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
Complete Growth Medium
Culture Medium: Iscove's modified Dulbecco's medium, 80%; fetal bovine serum,
20%.
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Culture Conditions
Temperature: 37.0°C
Isotype IgM
Name of Depositor USDA
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
References

Gamble HR, Graham CE. Monoclonal antibody-purified antigen for the immunodiagnosis of trichinosis. Am. J. Vet. Res. 45: 67-74, 1984. PubMed: 6703457

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Gamble HR, Graham CE. Monoclonal antibody-purified antigen for the immunodiagnosis of trichinosis. Am. J. Vet. Res. 45: 67-74, 1984. PubMed: 6703457