HT-2 clone A5E (ATCC® CRL-1841)

Organism: Mus musculus, mouse  /  Cell Type:: IL-2 dependent T lymphocyte  /  Tissue: spleen  / 

Organism Mus musculus, mouse
Tissue spleen
Cell Type IL-2 dependent T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain BALB/c
Applications

The line is dependent on IL-2 for growth and can be used to measure IL-2 activity in a very rapid and sensitive assay.

The cells are commonly used for characterization of lymphokines and cytokines.


Derivation
The HT-2 cell line was derived in 1979 by J. Watson at the University of Auckland from a culture of BALB/c spleen cells activated by sheep erythrocytes in the presence of IL-2.
Antigen Expression
Thy-1
Receptor Expression
interleukin 2 (IL-2)
Comments
The A5E clone was selected to provide a homogeneous population that is highly sensitive to IL-2.

In addition to IL-2, substantial proliferation is seen in the presence of interleukin 4 (interleukin-4, IL-4, also known as BSF-1).


Complete Growth Medium The complete medium for culturing CRL-1841 is composed of:
399.5 mL RPMI-1640 (ATCC 30-2001)
50 mL FBS (ATCC 30-2020)
0.5 mL 2-Mercaptoethanol (Gibco 21985-023)
50 mL Rat T-STIM with Con A (Thermo Fisher CB-40115)
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Subcuture every two days at 3.5 x 10 4 viable cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Name of Depositor JF Weaver, A Creasey
Year of Origin 1979
References

Kupper T, et al. Growth of an interleukin-2/interleukin 4-dependent T cell line induced by granulocyte-macrophage colony-stimulating factor (GM-CSF). J. Immunol. 138: 4288-4292, 1987. PubMed: 2953804

Ho SN, et al. Differential bioassay of interleukin-2 and interleukin-4. J. Immunol. Methods 98: 99-104, 1987. PubMed: 3494085

Lee F, et al. Isolation and characterization of a mouse interleukin cDNa clone that expresses B-cell stimulatory factor 1 activities and T-cell and mast-cell-stimulating activities. Proc. Natl. Acad. Sci. USA 83: 2061-2065, 1986. PubMed: 3083412

Watson J. Continuous proliferation of murine antigen-specific helper T lymphocytes in culture. J. Exp. Med. 150: 1510-1519, 1979. PubMed: 92524

. . J. Biol. Response Modif. 4: 96-109, 1985.

Kupper T, et al. Autocrine growth of T cells independent of interleukin 2: identification of interleukin 4 (IL 4, BSF-1) as an autocrine growth factor for a cloned antigen-specific helper T cell. J. Immunol. 138: 4280-4287, 1987. PubMed: 2953803

Woods A, et al. Granulocyte-macrophage colony stimulating factor produced by cloned L3T4a+, class II-restricted T cells induces HT-2 cells to proliferate. J. Immunol. 138: 4293-4297, 1987. PubMed: 2953805

Kotanides H, Reich NC. Interleukin-4-induced STAT6 recognizes and activates a target site in the promoter of the interleukin-4 receptor gene. J. Biol. Chem. 271: 25555-25561, 1996. PubMed: 8810328

Basic Documentation
References

Kupper T, et al. Growth of an interleukin-2/interleukin 4-dependent T cell line induced by granulocyte-macrophage colony-stimulating factor (GM-CSF). J. Immunol. 138: 4288-4292, 1987. PubMed: 2953804

Ho SN, et al. Differential bioassay of interleukin-2 and interleukin-4. J. Immunol. Methods 98: 99-104, 1987. PubMed: 3494085

Lee F, et al. Isolation and characterization of a mouse interleukin cDNa clone that expresses B-cell stimulatory factor 1 activities and T-cell and mast-cell-stimulating activities. Proc. Natl. Acad. Sci. USA 83: 2061-2065, 1986. PubMed: 3083412

Watson J. Continuous proliferation of murine antigen-specific helper T lymphocytes in culture. J. Exp. Med. 150: 1510-1519, 1979. PubMed: 92524

. . J. Biol. Response Modif. 4: 96-109, 1985.

Kupper T, et al. Autocrine growth of T cells independent of interleukin 2: identification of interleukin 4 (IL 4, BSF-1) as an autocrine growth factor for a cloned antigen-specific helper T cell. J. Immunol. 138: 4280-4287, 1987. PubMed: 2953803

Woods A, et al. Granulocyte-macrophage colony stimulating factor produced by cloned L3T4a+, class II-restricted T cells induces HT-2 cells to proliferate. J. Immunol. 138: 4293-4297, 1987. PubMed: 2953805

Kotanides H, Reich NC. Interleukin-4-induced STAT6 recognizes and activates a target site in the promoter of the interleukin-4 receptor gene. J. Biol. Chem. 271: 25555-25561, 1996. PubMed: 8810328