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CCD-1138Sk (ATCC® CRL-2707™)

Organism: Homo sapiens, human  /  Cell Type:: fibroblast  /  Tissue: skin  / 

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Organism Homo sapiens, human
Tissue skin
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age 43 years
Gender male
Ethnicity Caucasian
Applications
The cell line was derived from normal skin taken from the abdomen.
Storage Conditions liquid nitrogen vapor phase
Derivation
The cell line was derived from normal skin taken from the abdomen.
Clinical Data
male
Caucasian
43 years
Comments
The cell line was derived from normal skin taken from the abdomen.
Cells senesce after approximately 25 population doublings.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor P.Wood, ATCC
References

Cells from the current passage (2) have experienced approximately 4.5 population doublings beyond the biopsy material. The cells are capable of at least 13 additional population doublings before the onset of senescence.

skin taken from the abdomen

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
SDS
SDS
References

Cells from the current passage (2) have experienced approximately 4.5 population doublings beyond the biopsy material. The cells are capable of at least 13 additional population doublings before the onset of senescence.

skin taken from the abdomen