Lec1 [originally named Pro-5WgaRI3C] (ATCC® CRL-1735)

Organism: Cricetulus griseus, hamster, Chinese  /  Tissue: ovary  / 

Permits and Restrictions

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Organism Cricetulus griseus, hamster, Chinese
Tissue ovary
Product Format frozen
Morphology epithelial
Culture Properties loosely adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Gender female
Applications This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Derivation
This line is a mutant clone derived from the parental CHO clone Pro-5 (a proline auxotroph, see ATCC CRL-1781) by selection for resistance to wheat germ agglutinin.
Clinical Data
female
Comments
The cells lack GlcNAc glycosyl transferase so that N-linked carbohydrates are blocked at the Man5-GlcNAC2-Asn intermediate. The line may be useful for studying the effects of altered N-linked glycosylation on the function and compartmentalization of endogenous glycoproteins or glycoproteins introduced by viral infection or transfection of foreign DNA.
The population contains Pro+ revertants at low frequency (approximately 1 in 100,000 cells), and should be suitable for complementation studies which require the Pro- marker.
The cells are proline auxotrophs and must be grown in a medium that contains proline (40 mg/L).
The population contains Pro+ revertants at low frequency (approximately 1 in 100000 cells), and should be suitable for complementation studies which require the Pro- marker.
Complete Growth Medium Alpha minimum essential medium with ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10%
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Name of Depositor P Stanley
References

Stanley P, et al. Selection and characterization of eight phenotypically distinct lines of lectin-resistant Chinese hamster ovary cells. Cell 6: 121-128, 1975. PubMed: 1182798

Stanley P, et al. Chinese hamster ovary cells selected for resistance to the cytotoxicity of phytohemagglutinin are deficient in a UDP-N-acetylglucosamine-- glycoprotein N-acetylglucosaminyltransferase activity. Proc. Natl. Acad. Sci. USA 72: 3323-3327, 1975. PubMed: 1059116

Cross References

Nucleotide (GenBank) : U65792 Cricetulus griseus mutant N-acetylglucosaminyltransferase I mRNA, complete cds.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Stanley P, et al. Selection and characterization of eight phenotypically distinct lines of lectin-resistant Chinese hamster ovary cells. Cell 6: 121-128, 1975. PubMed: 1182798

Stanley P, et al. Chinese hamster ovary cells selected for resistance to the cytotoxicity of phytohemagglutinin are deficient in a UDP-N-acetylglucosamine-- glycoprotein N-acetylglucosaminyltransferase activity. Proc. Natl. Acad. Sci. USA 72: 3323-3327, 1975. PubMed: 1059116