mh [mocha] (ATCC® CRL-2709)

Organism: Mus musculus, mouse  /  Cell Type:: fibroblast  /  Tissue: muscle  /  Disease: Hermansky-Pudlak syndrome (HPS)

Organism Mus musculus, mouse
Tissue muscle
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Hermansky-Pudlak syndrome (HPS)
Age 1 to 3 days newborn
Storage Conditions lliquid nitrogen vapor phase
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: lliquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Name of Depositor AA Peden
References

Peden AA, et al. Assembly and function of AP-3 complexes in cells expressing mutant subunits. J. Cell Biol. 156: 327-336, 2002. PubMed: 11807095

. Mutation in AP-3 delta in the mocha mouse links endosomal transport to storage deficiency in platelets, melanosomes, and synaptic vesicles.. Neuron 21: 111-122, 1998. PubMed: 9697856

The mh cell line was derived from primary fibroblasts taken from muscle of homozygous mocha (mh/mh) mice. As a result of the mocha mutation, the fibroblasts are deficient in the delta subunit of the adaptor-like protein complex, AP-3. The cell line can be used to investigate both the assembly of AP-3 complexes and AP-3 function, and may serve as a model for Hermansky-Pudlak syndrome (HPS), a platelet storage pool deficiency syndrome.

Basic Documentation
References

Peden AA, et al. Assembly and function of AP-3 complexes in cells expressing mutant subunits. J. Cell Biol. 156: 327-336, 2002. PubMed: 11807095

. Mutation in AP-3 delta in the mocha mouse links endosomal transport to storage deficiency in platelets, melanosomes, and synaptic vesicles.. Neuron 21: 111-122, 1998. PubMed: 9697856

The mh cell line was derived from primary fibroblasts taken from muscle of homozygous mocha (mh/mh) mice. As a result of the mocha mutation, the fibroblasts are deficient in the delta subunit of the adaptor-like protein complex, AP-3. The cell line can be used to investigate both the assembly of AP-3 complexes and AP-3 function, and may serve as a model for Hermansky-Pudlak syndrome (HPS), a platelet storage pool deficiency syndrome.