BLO-11 (ATCC® CCL-198)

Organism: Mus musculus, mouse  /  Cell Type: fibroblast  / 

Permits and Restrictions

View Permits

Organism Mus musculus, mouse
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 30 days
Gender male
Applications
The line was derived from a mouse carrying the blotchy mutation.
The cells are deficient in lysyl oxidase.
ATCC CCL-197 is an age, sex and strain matched control for this line.
Tested and found negative for ectromelia virus (mousepox).
Derivation
The line was derived from a mouse carrying the blotchy mutation.
Clinical Data
male
Antigen Expression
H-2b
Genes Expressed
H-2b
Comments
The line was derived from a mouse carrying the blotchy mutation.
The cells are deficient in lysyl oxidase.
ATCC CCL-197 is an age, sex and strain matched control for this line.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.1% trypsin for about 10 minutes, and centrifuge to remove trypsin. Resuspend cell pellet in fresh medium and dispense into new flasks. Subculture every 7 days.
Name of Depositor RC Starcher, J Madaras
References

Starcher BC, et al. Lysyl oxidase deficiency in lung and fibroblasts from mice with hereditary emphysema. Biochem. Biophys. Res. Commun. 78: 706-712, 1977. PubMed: 20889

Starcher B, et al. Abnormal cellular copper metabolism in the blotchy mouse. J. Nutr. 108: 1229-1233, 1978. PubMed: 27591

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Starcher BC, et al. Lysyl oxidase deficiency in lung and fibroblasts from mice with hereditary emphysema. Biochem. Biophys. Res. Commun. 78: 706-712, 1977. PubMed: 20889

Starcher B, et al. Abnormal cellular copper metabolism in the blotchy mouse. J. Nutr. 108: 1229-1233, 1978. PubMed: 27591