Certified Reference Materials (CRMs)

  • SRM PCR-Based DNA Profiling Standard (ATCC® NIST-2391c)

    ATCC® Number: NIST-2391c

    Storage: Store in the dark between 2°C to 8°C. DO NOT FREEZE.
    Applications: For use in the standardization of forensic and paternity quality assurance procedures for polymerase chain reaction (PCR)-based genetic testing, for instructional law enforcement or non-clinical research purposes, and for quality assurance when assigning values to in-house control materials. It is not intended for any human or animal clinical diagnostic use.

    For-Profit:   $1,119.00 Non-Profit:   $1,119.00

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    Comments: SRM NIST-2391c is composed of well-characterized human deoxyribonucleic acid (DNA) in two forms: genomic DNA (Components A through D) and DNA to be extracted from cells that have been spotted onto 903 paper (Component E) and FTA paper (Component F).

     

    Component Description Amount Concentration(a)
    A Anonymous single-source female genomic DNA in TE-4 buffer 50 μL 1.1 – 2.1 ng/μL
    B Anonymous single-source male genomic DNA in TE-4 buffer 50 μL 1.1 – 2.1 ng/μL
    C Anonymous single-source male genomic DNA in TE-4 buffer 50 μL 1.1 – 2.1 ng/μL
    D Mixed-source (Components A and C) genomic DNA in TE-4 buffer 50 μL 1.1 – 2.1 ng/μL
    E Anonymous single-source female cells spotted on 903 paper Two 6 mm punches 7.5 × 104 cells per punch
    F Anonymous single-source male cells spotted on FTA paper Two 6 mm punches 7.5 × 104 cells per punch

     

    See the NIST Certificate of Analysis for directions for use and quality control specifications. 

    Certificate of Analysis

    NIST SDS

     

  • SRM Genomic DNA Standards for HER2 Measurements (ATCC® NIST-2373)

    ATCC® Number: NIST-2373

    Quantity: 100 µL of DNA solution per vial
    Applications: Used to assign ratios of the human epidermal growth factor 2 gene (HER2; the official gene symbol ERBB2) to unamplified reference genes. Measurements of the amplification (increased copies) of the HER2 gene in breast cancer samples are used as a biomarker for determining the classification for breast cancers

    For-Profit:   $1,473.00 Non-Profit:   $1,473.00

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    Product Format: 100 µL of DNA solution per vial
    Comments: SRM NIST-2373 consists of genomic DNA extracted from five breast cancer cell lines with different amounts of amplification of the HER2 gene. The five purified genomic DNAs were solubilized in a buffer consisting of 10 mmol/L tris(hydroxymethyl)aminomethane and 0.1 mmol/L ethylenediaminetetraacetic acid disodium salt (EDTA) pH 8.0 (TE–4). The five components are genomic DNA materials derived from human cell lines SK-BR-3, MDA-MB-231, MDA-MB-361, MDA-MB-453, and BT-474, labeled A, B, C, D, and E, respectively. A unit of SRM 2373 consists of five vials, one of each component, containing approximately 100 μL of DNA solution. Each of these vials is labeled and is sealed with a color-coded screw cap.

    See the NIST Certificate of Analysis for directions for use and quality control specifications. 

    Certificate of Analysis

    NIST SDS

  • MDA-MB-231 (ATCC® CRM-HTB-26)

    ATCC® Number: CRM-HTB-26

    Organism: Homo sapiens, human
    Cell Type: Epithelial
    Tissue: Mammary Gland/Breast; Derived From Metastatic Site: Pleural Effusion
    Disease Adenocarcinoma

    For-Profit:   $714.00 Non-Profit:   $535.50

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    Tissue: Mammary Gland/Breast; Derived From Metastatic Site: Pleural Effusion
    Product Format: frozen
  • Candida albicans (Robin) Berkhout (ATCC® CRM-10231)

    ATCC® Number: CRM-10231

    Deposited As Candida albicans (Robin) Berkhout, anamorph
    Alternate State: Monilia pinoyi (Castellani) Castellani et Chalmers; Endomyces albicans (Robin) Vuillemin; Candida claussenii Lodder et Kreger-van Rij, anamorph; Candida stellatoidea (Jones et Martin) Langeron et Guerra, anamorph
    Designation: 3147 [CBS 6431, CCY 29-3-106, CIP 48.72, DSM 1386, IFO 1594, NCPF 3179, NCYC 1363, NIH 3147, VTT C-85161]

    For-Profit:   $420.00 Non-Profit:   $315.00

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    Comments: Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.

    Certified Reference Material produced under an ISO Guide 34:2009 accredited process. For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation.

    For sterility testing, not more than five passages from the ATCC culture should be used.

    Type Strain: no
    Biological Safety Level: 1
  • Staphylococcus aureus subsp. aureus Rosenbach (ATCC® CRM-6538)

    ATCC® Number: CRM-6538

    Strain Designations: FDA 209
    Type Strain: no
    Biosafety Level: 2

    For-Profit:   $420.00 Non-Profit:   $315.00

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    Biosafety Level: 2
    Product Format: freeze-dried
  • High molecular weight genomic DNA isolated from PSN-1; Pancreas Adenocarcinoma; Human cell line. (ATCC® CRM-CRL-3211D)

    ATCC® Number: CRM-CRL-3211D

    Quantity: 5 μg
    Storage: -80°C or colder
    Applications: For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. ISO Guide 34:2009.

    For-Profit:   $408.00 Non-Profit:   $408.00

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    Product Format: frozen 5 μg
    Comments:

    Store at - 80°C or colder in a manual defrost freezer.  Avoid freeze-thawing.  Alternatively, product can be stored at -20°C or colder, but stability at this temperature has not been tested by the ATCC and is not guaranteed.

    Certified Reference Material produced under an ISO Guide 34:2009 accredited process.

    This item has been tested for KRAS mutation: Homozygous DNA Change: c.34G>C (correlates to protein sequence p.G12R).


  • NCI-H441 [H441] (ATCC® CRM-HTB-174)

    ATCC® Number: CRM-HTB-174

    Organism: Homo sapiens, human
    Cell Type: Epithelial Cell (Kras Crm)
    Tissue: Lung
    Disease Papillary Adenocarcinoma

    For-Profit:   $714.00 Non-Profit:   $535.50

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    Tissue: Lung
    Product Format: frozen
  • SRM Human DNA Quantitation Standard (ATCC® NIST-2372)

    ATCC® Number: NIST-2372

    Quantity: 110 µL of DNA solution per vial
    Storage: Store in the dark between 2°C to 8°C. Do not freeze
    Applications: forensic quantitation

    For-Profit:   $811.00 Non-Profit:   $811.00

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    Product Format: 110 µL of DNA solution per vial
    Comments: SRM NIST-2372 is intended primarily for use in the value assignment of human genomic DN forensic quantitation materials. It is not intended for any human or animal clinical diagnostic use. SRM 2372 consists of three well-characterized human genomic DNA materials solubilized in 10 mmol/L 2-amino-2-(hydroxymethyl)-1,3-propanediol hydrochloride (Tris HCl) and 0.1 mmol/L ethylenediaminetetraacetic acid disodium salt (disodium EDTA) using deionized water adjusted to pH 8.0 buffer (TE-4). The three component genomic DNA materials, labeled A, B, and C, are derived from a single male donor, multiple female donors, and multiple male and female donors, respectively. A unit of the SRM consists of one sterile 2 mL vial of each component, each vial containing approximately 110 μL of DNA solution. Each of these vials is labeled and is sealed with a color–coded screw cap.

    See the NIST Certificate of Analysis for directions for use and quality control specifications. 

    Certificate of Analysis

    NIST SDS


  • HuT 78 (ATCC® CRM-TIB-161)

    ATCC® Number: CRM-TIB-161

    Organism: Homo sapiens, human
    Cell Type: Cutaneous T Lymphocyte (Kras Crm)
    Disease Sezary Syndrome

    For-Profit:   $714.00 Non-Profit:   $535.50

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  • Acholeplasma laidlawii (Sabin) Edward and Freundt (ATCC® qCRM-23206D)

    ATCC® Number: qCRM-23206D

    Strain Designations: Genomic DNA from Acholeplasma laidlawii strain PG8 [NCTC 10116, PG8; A]
    Type Strain: yes
    Biosafety Level: 1

    For-Profit:   $490.00 Non-Profit:   $490.00

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    Biosafety Level: 1
    Product Format: frozen
    Specification range: 1x106 - 1x107 genome copies/µL