Dehalococcoides sp. (ATCC® BAA-2099)

Strain Designations: GT  /  Type Strain: no  /  Biosafety Level: 1

Strain Designations GT
Isolation
Chloroethene contaminated aquifer; Cottage Grove, WI, USA
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genome Sequenced Strain

Dehalococcoides sp. GT, complete genome

Type Strain no
Comments
Genome sequenced strain
Growth can best be monitored using real time PCR; see Protocol for quantifying in Related Links.
Medium ATCC® Medium 2760: MOPS Buffered Medium for Dehalococcoides sp.
Growth Conditions
Temperature: 24°C to 30°C
Atmosphere: Anaerobic gas mixture, 100% N2 or 80% N2-20% CO2
Name of Depositor FE Löffler
Isolation
Chloroethene contaminated aquifer; Cottage Grove, WI, USA
References

Moench TT, Zeikus JG. An improved preparation method for a titanium (III) media reductant. J. Microbiol. Methods. 1: 199-202, 1983.

Sung Y, et al. Quantitative PCR confirms purity of strain GT, a novel trichloroethene-to-ethene-respiring Dehalococcoides isolate. Appl. Environ. Microbiol. 72(3): 1980-1987, 2006. PubMed: 16517646

Ritalahti KM, et al. Quantitative PCR targeting 16S rRNA and reductive dehalogenase genes simultaneously monitors multiple Dehalococcoides strains. Appl. Environ. Microbiol. 72(4): 2765-2774, 2006. PubMed: 16597981

Cross References

Nucleotide (GenBank) : CP001924 Dehalococcoides sp. GT, complete genome