HECA-452 (ATCC® HB-11485)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

View Permits

Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain: Wistar

Applications
The antibody reacts with an antigen on skin homing memory T cells.
It binds a T cell carbohydrate ligand for vascular E-selectin.
Storage Conditions liquid nitrogen vapor phase
Genes Expressed
immunoglobulin; monoclonal antibody; against human cutaneous lymphocyte antigen (CLA)
Cellular Products
immunoglobulin; monoclonal antibody; against human cutaneous lymphocyte antigen (CLA)
Comments
The antibody reacts with an antigen on skin homing memory T cells.
It binds a T cell carbohydrate ligand for vascular E-selectin.
The antibody also recognizes sialyl Lewis x and sialyl Lewis a antigens and other carbohydrate ligands for human E-selectin.
Complete Growth Medium RPMI 1640 medium with 0.05 mM 2-mercaptoethanol and non essential amino acids, 90%; fetal bovine serum, 10%
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: culture medium, 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Isotype rat IgM
Name of Depositor Stanford University
U.S. Patent Number
References

Butcher EC, Picker LJ. Neutrophil LECAM-1 as indicator of neutrophil activation. US Patent 5,316,913 dated May 31 1994

Duijvestijn AM, et al. High endothelial differentiation in human lymphoid and inflammatory tissues defined by monoclonal antibody HECA-452. Am. J. Pathol. 130: 147-155, 1988. PubMed: 3276207

Berg EL, et al. The cutaneous lymphocyte antigen is a skin lymphocyte homing receptor for the vascular lectin endothelial cell-leukocyte adhesion molecule 1. J. Exp. Med. 174: 1461-1466, 1991. PubMed: 1720810

The antibody reacts with a 200-kd cell-surface glycoprotein present on a subset of peripheral blood T cells, including both CD4+ and CD8+ T cells.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Butcher EC, Picker LJ. Neutrophil LECAM-1 as indicator of neutrophil activation. US Patent 5,316,913 dated May 31 1994

Duijvestijn AM, et al. High endothelial differentiation in human lymphoid and inflammatory tissues defined by monoclonal antibody HECA-452. Am. J. Pathol. 130: 147-155, 1988. PubMed: 3276207

Berg EL, et al. The cutaneous lymphocyte antigen is a skin lymphocyte homing receptor for the vascular lectin endothelial cell-leukocyte adhesion molecule 1. J. Exp. Med. 174: 1461-1466, 1991. PubMed: 1720810

The antibody reacts with a 200-kd cell-surface glycoprotein present on a subset of peripheral blood T cells, including both CD4+ and CD8+ T cells.