NCI-H2195 [H2195] ATCC ® CRL-5931™ Homo sapiens lung; derive

Permits and Restrictions	View Permits View Restrictions
Organism	Homo sapiens, human
Tissue	lung; derived from metatstatic site: bone marrow
Cell Type	lymphoblast
Product Format	frozen
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	stage E, carcinoma; small cell lung cancer
Age	67 years
Gender	male
Ethnicity	Caucasian
Storage Conditions	liquid nitrogen vapor phase

Derivation	The line was established in June 1989 from bone marrow metastasis of a lung.
Clinical Data	67 years Caucasian male
Comments	A lymphoblastoid line from the same patient is available as ATCC CRL-5956.

Complete Growth Medium	HITES medium supplemented with 5% fetal bovine serum The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium 0.005 mg/ml Insulin 0.01 mg/ml Transferrin 30nM Sodium selenite (final conc.) 10 nM Hydrocortisone (final conc.) 10 nM beta-estradiol (final conc.) extra 2mM L-glutamine (for final conc. of 4.5 mM) 5% fetal bovine serum (final conc.)
Subculturing	Remove medium, rinse with 0.25% (w/v) trypsin - 0.53 mM EDTA solution Add 1 to 2 mL additional trypsin solution and allow flasks to remain at room temperature (or incubate at 37°C) until cells detach. Resuspend pellet in fresh medium and dispense into a culture flask. Subcultivation Ratio: A ratio of 1:2 to 1:3 is recommended Medium Renewal: Twice weekly
Cryopreservation	Freeze medium: Culture medium, 90%; DMSO, 5%; FBS ,5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C

STR Profile	Amelogenin: X,Y CSF1PO: 11 D13S317: 11 D16S539: 10 D5S818: 12 D7S820: 10,11 THO1: 9.3 TPOX: 8,9 vWA: 18

Name of Depositor	AF Gazdar, JD Minna
Year of Origin	1989
References	NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. 100 pack years The line was established from tissue taken from a patient with a 100 pack-year history of smoking.

Name of Depositor

AF Gazdar, JD Minna

Year of Origin

1989

References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

100 pack years

The line was established from tissue taken from a patient with a 100 pack-year history of smoking.

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis SDS
Restrictions	The line is available with the following restrictions: This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. Any proposed commercial use of the these cells, or their products, must first be negotiated with the National Cancer Institute (NCI). For further information, please contact NCI’s Technology Transfer Center at NCI_TTC_Contact@mail.nih.gov or by phone at (240)-276-5514.
References	NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. 100 pack years The line was established from tissue taken from a patient with a 100 pack-year history of smoking.

NCI-H2195 [H2195] (ATCC^® CRL-5931^™)