Gap Per (ATCC® CRL-1335)

Organism: Homo sapiens, human  /  Tissue: skin  /  Disease: Ehlers-Danlos syndrome, possible heterozygote

Organism Homo sapiens, human
Tissue skin
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Ehlers-Danlos syndrome, possible heterozygote
Age 66 years
Gender male
Clinical Data
The tissue donor was a possible heterozygote for Ehlers-Danlos syndrome, and was the grandfather of Cor Per II (ATCC CRL-1243), No Per (ATCC CRL-1327) and Jo Per (ATCC CRL-1332).
male
Comments
The tissue donor was a possible heterozygote for Ehlers-Danlos syndrome, and was the grandfather of Cor Per II (ATCC CRL-1243), No Per (ATCC CRL-1327) and Jo Per (ATCC CRL-1332).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 8 to 10 days.