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Trichormus variabilis Komarek and Anagnostidis

29413

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Trichormus variabilis is a cyanobacterium that was isolated in 1964 from freshwater in Mississippi. This whole-genome sequenced strain has applications in biotechnology and is known to produce heterocysts.
Product category
Bacteria
Classification

Cyanobacteria, Nostocales

Strain designation
[IUCC 1444; MSU A-37]
Type strain
No
Genome sequenced strain
Yes
Isolation source
Freshwater
Geographical isolation
United States; Mississippi
Applications
Bioinformatics
Product format
Freeze-dried
Storage conditions
2°C to 8°C
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Biotechnology
Preceptrol
No

Characteristics

Comments
Glycogen accumulation and the induction of nitrogenase activity
Inhibition of nitrogen fixation by nitrite
Isolation of cyanobacterial heterocysts
Plating
Heterocysts
Regulation of electron transport to nitrogenase
Uv-inducible DNA repair
Genome sequenced strain

Handling information

Medium
Temperature
26°C
Incubation
Under light intensity of 2,000-3,000 lux
Handling procedure
  1. Open vial according to enclosed instructions.
  2. Using a single tube of #616 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette.  Rehydrate the entire pellet. 
  3. Aseptically transfer this aliquot back into the broth tube.  Mix well.
  4. Use several drops of the suspension to inoculate a second tube of broth and a #616 slant. 
  5. Incubate tubes at 26°C under 2000-3000 LUX light.  It is helpful to incubate test tubes in a slanted position to increase gas exchange in broth and to enhance exposure to light. 
Handling notes
Good growth, indicated by increased pigmentation in the broth or on the slant, should occur after two weeks of incubation.  Examine cells microscopically to assure that they are intact and healthy.  At this time additional test tubes or flasks can be inoculated.   A 5% inoculum is recommended  (i.e. 5 ml of culture to 100 ml fresh medium).

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Anabaena variabilis Kutzing
Depositors
CP Wolk
Chain of custody
ATCC <-- CP Wolk <-- IUCC 1444 (Anabaena flos-aquae) <-- R. Tischer MSU A-37
Type of isolate
Environmental
Year of origin
1964
Cross references
GenBank CP000117 Anabaena variabilis ATCC 29413, complete genome.
GenBank X66864 A.variabilis psaI gene.
GenBank Y13044 A.variabilis dnaK gene.
GenBank Z46887 A.variabilis fdxH1 gene.
GenBank AJ132703 Anabaena variabilis apd gene.
GenBank AJ271820 Anabaena variabilis petG gene.
GenBank AJ299220 Anabaena variabilis petM gene.
GenBank AJ132704 Anabaena variabilis dnaJ1 gene.
GenBank X93923 A.variabilis psaF and psaJ genes.
GenBank X06210 Anabaena variabilis ferredoxin gene.
GenBank X14343 Anabaena petF1 gene for ferredoxin I.
GenBank AJ271821 Anabaena variabilis partial petN gene.
GenBank Z46890 A.variabilis fdxB, fdxH2 and nifW2 genes.
GenBank AJ132705 Anabaena variabilis dnaJ2 gene and ORF1.
GenBank M28152 A.variabilis nifD gene 5' recombination site.
GenBank AJ132706 Anabaena variabilis ORF3 (partial) and ORF2.
GenBank AH000860 A.variabilis nifD gene 5' recombination site.
GenBank AJ005201 Anabaena variabilis cphB and cphA genes, complete.
GenBank AJ292758 Anabaena variabilis susA gene for sucrose synthase.
GenBank X73824 A.variabilis gene for vanadium-dependent nitrogenase.
GenBank X87934 A.variabilis internal part of a 5.1 kbp EcoRI fragment.
GenBank M29680 A.variabilis DNA-repair protein (recA) gene, complete cds.
GenBank X79285 A.variabilis (ATCC 29413) hoxF, hoxU, hoxY and hoxH genes.
GenBank L47327 Anabaena variabilis transaldolase (tal) gene, complete cds.
GenBank U38478 Anabaena variabilis cytochrome P450 (cyp110A2) gene, complete cds.
GenBank AJ003195 Anabaena variabilis devB, devC and devA genes of ABC transporter.
GenBank M29074 A.variabilis excised nifD element circular DNA, recombination site.
GenBank M28153 A.variabilis nifD gene 3' recombination site, and xisA gene, 5' end.
GenBank X69898 A.variabilis nifB gene (partial), fdxN gene, and nifS gene (partial).
GenBank U89346 Anabaena variabilis dinitrogenase reductase (nifH) gene, complete cds.
GenBank AJ311361 Anabaena variabilis petC3 gene for short putative Rieske-FeS protein.
GenBank Y13216 Anabaena variabilis hupS, hupL genes and 5 unknown open reading frames.
GenBank L26346 Anabaena variabilis ferrdoxin NADP oxidoreductase (petH) gene, complete cds.
GenBank L26326 Anabaena variabilis reaction center protein (psaA and psaB) genes, complete cds.
GenBank U89516 Anabaena variabilis nitrogen-responsive regulatory protein (ntcA), complete cds.
GenBank AF301148 Anabaena variabilis putative histidine protein kinase (hoxJ) gene, complete cds.
GenBank U25173 Anabaena variabilis nitrogenase 2 alpha subunit (nifD2) gene, 3' region, partial cds.
GenBank AJ271819 Anabaena variabilis petC gene for Rieske FeS-protein and petA gene for cytochrome f.
GenBank L07497 Anabaena variabilis glyceraldehyde-3-phosphate dehydrogenase (gap1) gene, complete cds.
GenBank L07498 Anabaena variabilis glyceraldehyde-3-phosphate dehydrogenase (gap2) gene, complete cds.
GenBank L07499 Anabaena variabilis glyceraldehyde-3-phosphate dehydrogenase (gap3) gene, complete cds.
GenBank AF301157 Anabaena variabilis heterocyst differentiation protein HetR (hetR) gene, complete cds.
GenBank L20472 Anabaena variabilis vanadium-dependent nitrogenase (vnfDG and vnfK) genes, complete cds's.
GenBank U25172 Anabaena variabilis nitrogenase 2 alpha subunit (nifD2) gene, central region, partial cds.
GenBank AJ271818 Anabaena variabilis petB gene for cytochrome b6 and petD gene for b6f complex subunit IV.
GenBank AH006561 Anabaena variabilis nitrogenase 2 alpha subunit (nifD2) gene, central region, partial cds.
GenBank M29073 Anabaena variabilis recombined functional nifD gene encoding alpha nitrogenase, partial cds.
GenBank U25160 Anabaena variabilis dinitrogenase reductase (nifH2) gene, 5' upstream region and partial cds.
GenBank U51863 Anabaena variabilis vanadium nitrogenase associated proteins VnfE (vnfE) and VnfN (vnfN) genes, complete cds.
GenBank AJ311360 Anabaena variabilis petC2 gene for putative Rieske-FeS protein and partial ntr gene for NADP-thioredoxin reductase.
GenBank U49859 Anabaena variabilis Mo-nitrogenase operon NifB2, NifS2, NifU2, dinitrogenase reductase (nifH2), dinitrogenase alpha subunit (nifD2), dinitrogenase beta subunit (nifK2), Orf1 protein, NifEN2, NifX2, Orf2 protein, Orf3 protein, and NifW2 genes,

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Schrautemeier B, et al. In vitro studies on pathways and regulation of electron transport to nitrogenase with a cell-free extract from heterocysts of Anabaena variabilis. Arch. Microbiol. 137: 14-20, 1984.

Wolk CP, Wojciuch E. Simple methods for plating single vegetative cells of, and for replica- plating, filamentous blue-green algae. Arch. Mikrobiol. 91: 91-95, 1973. PubMed: 4197882

Boehme H. Inhibition of nitrogen fixation by nitrite in Anabaena variabilis. Arch. Microbiol. 146: 99-103, 1986.

Jensen BB, et al. Isolation of cyanobacterial heterocysts with high and sustained dinitrogen-fixation capacity supported by endogenous reductants. Arch. Microbiol. 145: 241-247, 1986. PubMed: 3094473

Levine E, Thiel T. UV-inducible DNA repair in the cyanobacteria Anabaena spp. J. Bacteriol. 169: 3988-3993, 1987. PubMed: 3114232

View All Curated Citations for this Product

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