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pUCSV40-CPC/MEN-1 [molecular clone of Simian virus 40, SV-40]

VRMC-4

Organism
Simian virus 40, Simian virus 40
Classification
Polyomaviridae, Polyomavirus
Clone type
Clone
Isolation source
Contains a full-length genome of SV-40 strain CPC/MEN cloned into the EcoRI site of pUC-19. SV40-CPC/MEN was isolated and cloned from virus stock derived from choroid plexus carcinoma DNA sample 12 (from 4-year-old child).
Applications
Molecular biology
Product format
Freeze-dried
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
contains sequence

Characteristics

Comments
It has an archetypal viral regulatory region and was tested by lipofection of EcoRI-linearized plasmid DNA into TC7 cells, with cells showing subsequent production of typical SV-40 CPE. Lipofection into TC7 and Butel lab CV-1 cells was verified.
Contains a full-length genome of SV-40 strain CPC/MEN cloned into the EcoRI site of pUC-19. SV40-CPC/MEN was isolated and cloned from virus stock derived from choroid plexus carcinoma DNA sample 12 (from a 4 yr old child).
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
7.86
Intact vector size
2.686
Vector name
pUC-19
Type of vector
E. coli

Insert information

Insert size (kb)
5.1799999999999997
Type of DNA
cDNA
Insert source
choroid plexus carcinoma
Insert tissue
choroid plexus carcinoma
Gene product
[SV40-CPC/MEN-1]

Handling information

Host
Escherichia coli
Medium
Temperature
37°C
Handling notes
This variant of SV40-CPC/MEN has an archetypal viral regulatory region and was tested by lipofection of EcoR1-linearized plasmid DNA into TC7 cells, with cells showing subsequent production of typical SV40 CPE. Lipofection into TC7 and Butel Lab CV-1 cells was repeated and verified by DNA sequence analysis of the resulting virus stocks.

DEPOSITED ON BEHALF OF:  Dr. J.A. Lednicky

History

Deposited as
Simian virus 40
Depositors
JA Lednicky, JS Butel
Special collection
NCRR Contract
Cross references
GenBank AF156108

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Stewart AR, et al. Sequence analyses of human tumor-associated SV40 DNAs and SV40 viral isolates from monkeys and humans. J. Neurovirol. 4: 182-193, 1998. PubMed: 9584955

Lednicky JA, et al. Natural simian virus 40 strains are present in human choroid plexus and ependymoma tumors. Virology 212: 710-717, 1995. PubMed: 7571441

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