Suid herpesvirus 1 (ATCC® VR-2116)

Classification: Herpesviridae, Varicellovirus  /  Product Format: frozen

Permits and Restrictions

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Classification Herpesviridae, Varicellovirus
Deposited As Pseudorabies virus
Agent Suid herpesvirus 1
Common Name Pseudorabies virus
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
derived from PRV dl tk (Kit), ATCC VR-2074
Product Format frozen
Storage Conditions -70°C or colder
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Comments
Live marker vaccine of PRV attenuated by deletions in thymidine kinase gene. Also has deletion of PRV g92 (gIII) gene.
Cultivate virus at an m.o.i. of 0.01 PFU/ml in rab cell culture at 34.5C. Harvest. Sonicate prior to infecting new cells. Virus replicates at 39C but does not induce thymidine kinase activity or a late glycoprotein due to deletion mutations.
Effect on Host
Cytopathic effects in tissue culture
Recommended Host
rabbit cells
swine testicle cells; RAB-9 cells (ATCC ); cultures of bovine, murine, canine, feline or porcine cells
Growth Conditions
Incubation:: 3 days at 30°C or 39°C
Effect on Host
Cytopathic effects in tissue culture
Name of Depositor NovaGene, Inc., Baylor College of Medicine
U.S. Patent
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Source
derived from PRV dl tk (Kit), ATCC VR-2074
References

Kit S, et al. Second-generation pseudorabies virus vaccine with deletions in thymidine kinase and glycoprotein genes. Am. J. Vet. Res. 48: 780-793, 1987. PubMed: 3035972

Kit M, Kit S. Pseudorabies virus mutants, vaccines containing same, methods for the production of same and methods for the use of same. US Patent 4,711,850 dated Dec 8 1987

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
  • This item is listed on the Commerce Control List of the U.S. Department of Commerce and is distributed only within the 50 United States. Distribution requires completion of a Customer Acceptance of Responsibility (CAR) for Commerce Control List (CCL) Biologicals form.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation