Human herpesvirus 1 (ATCC® VR-1789)

Classification: Herpesviridae, Simplexvirus  /  Product Format: frozen 1 mL per vial

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Classification Herpesviridae, Simplexvirus
Agent Human herpesvirus 1
Strain ATCC-2011-9
Common Name Herpes simplex virus 1
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-1789 images
Comments This virus was isolated in cell culture at ATCC.
Effect on Host CPE, refractile cell rounding
Recommended Host Vero (ATCC® CCL-81™)
Growth Conditions Temperature: 37°C

Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 80-90% confluent.  Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted in VGM to provide an optimal MOI (e.g. MOI 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding VGM.

Incubation: 2-4 days
Effect on Host CPE, refractile cell rounding
Name of Depositor ATCC
Source Clinical patient positive for HSV-1 in Virginia, July 2011
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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