Influenza B virus (ATCC® VR-1735)

Classification: Orthomyxoviridae, Influenzavirus B  /  Product Format: frozen 1 mL per vial

Classification Orthomyxoviridae, Influenzavirus B
Agent Influenza B virus
Strain B/Taiwan/2/62
Applications
Emerging infectious disease research
Respiratory research
Influenza Research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-1735 images
Comments
Derived by adaptation from allantoic product ATCC® VR-295™ to MDCK cells (ATCC® CCL-34™). ATCC® VR-295™ and ATCC® VR-1735™ have not been compared for sequence or infectivity in chicken embryo culture and tissue culture.
Effect on Host
CPE, rounding and degeneration
Recommended Host
MDCK (ATCC® CCL-34™)
Growth Conditions
Temperature: 33°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days
Effect on Host
CPE, rounding and degeneration
Name of Depositor RQ Robinson, Centers for Disease Control and Prevention; ATCC®
Special Collection ATCC
Source Patient in Taiwan, 1962
References

Green IJ, et al. The isolation and characterization of a new Influenza Type B virus on Taiwan. Am. J. Hyg. 79: 107-112, 1964. PubMed: 14114349