Influenza A virus (H3N2) (ATCC® VR-1680)

Classification: Orthomyxoviridae, Influenzavirus A  /  Product Format: frozen 1 mL per vial

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Classification Orthomyxoviridae, Influenzavirus A
Agent Influenza A virus (H3N2)
Strain A/Aichi/2/68
Applications
Emerging infectious disease research
Respiratory research
Influenza Research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments
Derived by adaptation from allantoic product ATCC® VR-547™ to MDCK cells (ATCC® CCL-34™). ATCC® VR-547™ and ATCC® VR-1680™ have not been compared for sequence or infectivity in chicken embryo culture and tissue culture.
Effect on Host
CPE, rounding and degeneration
Recommended Host
MDCK (ATCC® CCL-34™)
Growth Conditions
Temperature: 35°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days
Effect on Host
CPE, rounding and degeneration
Name of Depositor MT Coleman, Centers for Disease Control and Prevention; ATCC®
Source Isolated from sailor on Israeli ship docking in Aichi, Japan, 1968
References

Coleman MT, et al. The Hong Kong-68 influenza A2 variant. Lancet 2: 1384-1386, 1968. PubMed: 4177941

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation