Primary Cord Blood CD34+ Cells, Normal, Human (ATCC® PCS-800-014)

Organism: Homo sapiens, human  /  Tissue: Umbilical  /  Cell Type: Hematopoietic stem

Permits and Restrictions

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Organism Homo sapiens, human
Tissue Umbilical
Cell Type Hematopoietic stem
Morphology Spherical; variable after culturing
Growth Properties Suspension; variable after culturing
Biosafety Level 1

 [These primary cells are not known to harbor an agent recognized to cause disease in healthy adult humans. Handle as a potentially biohazardous material under at least Biosafety Level 1 containment. Cells derived from primate lymphoid tissue may fall under the regulations of 29 CFR 1910.1030 Bloodborne Pathogens. 

ATCC recommends that appropriate safety procedures be used when handling all primary cells and cell lines, especially those derived from human or other primate material. Detailed discussions of laboratory safety procedures are provided in Laboratory Safety: Principles and Practice, 2nd ed. (ASM Press, Washington, DC) (Fleming et al., 1995) and Caputo, J.L. Biosafety procedures in cell culture. (1988) J. Tissue Culture Methods 11:223. Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.] 

Human Material Precaution 

All tissues used for isolation are obtained under informed consent and conform to HIPAA standards to protect the privacy of the donor’s personal health information. It is best to use caution when handling any human cells. We recommend that all human cells be accorded the same level of biosafety consideration as cells known to carry HIV. With infectious virus assays or viral antigen assays, even a negative test result may leave open the possible existence of a latent viral genome.


Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Normal
Age Lot Specific
Gender Lot Specific
Ethnicity Lot Specific
Applications Applications for use include the study of stem cell biology, hematology, transplantation, and oncology.
Product Format frozen 0.50 mL
Storage Conditions -130°C
Comments Cluster of differentiation 34 (CD34) is a sialomucin-family protein that is expressed on the surface of blood, endothelial, and bone marrow-derived progenitor cells. While the functions of CD34 are not well understood, there is evidence that the protein is involved in maintaining progenitor cells in an undifferentiated state.
The ATCC maintains CD34+ bone marrow-derived and CD34+ cord blood-derived cells which may be used to support physiologically relevant research in regenerative medicine, transplant studies, stem cell biology, hematology, and oncology. These pluripotent stem cells are isolated by immunomagnetic positive selection. In addition, the ATCC Cell Systems Laboratory has conducted in-depth characterization of the cells including differentiation into all three cell lineages.
Complete Growth Medium CD34+ cells have a limited lifespan in culture and should only be thawed immediately prior to their intended use. ATCC does not recommend maintaining CD34+ cells in culture in the absence of application-specific growth factors.
Subculturing N/A
Volume 0.50 mL
Cells per Vial One vial contains a minimum of 5 x 105  viable cells.
Viral Testing
Hepatitis B: Negative
Hepatitis C: Negative
HIV(I/II): Negative
HTLV(I/II): Negative
WNV: Negative
Trypanasome: Negative
Viability ≥ 70% when thawed from cryopreservation
Population Doubling Capacity N/A
C of A
Certificate of Analysis
Certificate of Analysis
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
FAQs
  1. Cryopreservation of PCS-800-014 Cord Blood Cells
    ATCC CD34+ cord blood cells are cryopreserved in CryoStor
    Date Updated: 11/13/2014
  2. Isolation of PCS-800-014 Cord Blood cells
    These CD34+ cells are isolated using positive immunomagnetic cell separation from umbilical cord blood.
    Date Updated: 11/13/2014
  3. Purity of PCS-800-014 Cord Blood Cells
    ATCC CD34+ cells have been tested to have a purity of over 90% as measured by flow cytometry with a monoclonal anti-CD34 antibody.
    Date Updated: 11/13/2014
  4. Donor source for PCS-800-014 Cord Blood Cells
    No, a single ATCC CD34+ cryovial only contains cells from a single donor. The Certificate of Analysis available on the ATCC website will list available donor information for each lot of cell...
    Date Updated: 11/13/2014
  5. Culture of PCS-800-014 Cord Blood Cells
    CD34+ cord blood cells have a limited lifespan in culture and should be thawed immediately prior to their intended experimental applications. ATCC does not recommend maintaining CD34+ cells ...

    Date Updated: 11/14/2014
  6. PCS-800-014 cord blood cells from non-normal donors
    No, at this time ATCC does not offer CD34+ cells from non-normal donors.

    Date Updated: 11/14/2014
  7. Post-thaw yield and viability of PCS-800-014
    We recommend the following thawing procedure:
    Date Updated: 11/14/2014
  8. Differentiation capacity of PCS-800-014 cord blood cells


    Date Updated: 2/18/2015