Dermal Fibroblasts; Normal, Human, Neonatal, Mitomycin C Treated (ATCC® PCS-201-011)

Organism: Homo sapiens, human  / 

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Organism Homo sapiens, human
Morphology Cobblestone appearance; cells are rounded, not flat; cells display a high mitotic index; at near 80% confluence, the cells will be associated with each other in colonies.
Growth Properties Adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age Neonatal
Gender Male
Applications
The cells can be used as a feeder layer to support the growth of embryonic stem (ES) cells and for the maintenance of ES cells in the undifferentiated state. Suitable for use with other cell types that require a feeder layer for optimal attachment and proliferation.
Product Format frozen 1 mL
Storage Conditions -130°C or below
Comments
Primary neonatal fibroblasts have been treated with mitomycin C at passage #1 and will not replicate. Once the feeder cells have attached, the culture medium should be changed to accommodate the cells to be supported.
Complete Growth Medium
1. Obtain one growth kit from the freezer; make sure that the caps of all containers are tight.
2. Thaw the components of the growth kit just prior to adding them to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath, and shake to dissolve any precipitates prior to adding to the basal medium.
3. Obtain one bottle of Fibroblast Basal Medium (480 mL) from cold storage.
4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
5. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the volume of each growth kit component, as indicated in either Table 1 or 2, to the bottle of basal medium using a separate sterile pipette for each transfer.
Antimicrobials and phenol red are not required for proliferation, but may be added if desired. The recommended volume of each optional component to be added to the complete growth media is summarized in Table 3.
6. Tightly cap the bottle of complete growth medium and swirl the contents gently to assure a homogeneous solution. Do not shake forcefully to avoid foaming. Label and date the bottle.
7. Complete growth media should be stored in the dark at 2°C to 8°C (do not freeze). When stored under these conditions, complete growth media is stable for 30 days.
Volume 1 mL
Sterility Tests
Bacteria and Yeasts: Negative.
Mycoplasma: Negative
Viral Testing
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Viability ≥50% when thawed from cryopreservation
C of A
Certificate of Analysis
Certificate of Analysis
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation