Vascular Cell Basal Medium

Endothelial cells derived from large vessels

• Primary Umbilical Vein Endothelial Cells; Normal, Human, ATCC PCS-100-010
• Primary Umbilical Vein Endothelial Cells; Normal, Human, Pooled, ATCC PCS-100-013
• Primary Aortic Endothelial Cells; Normal, Human, ATCC PCS-100-011
• Primary Coronary Artery Endothelial Cells; Normal, Human, ATCC PCS-100-020
• Primary Pulmonary Artery Endothelial Cells; Normal, Human, ATCC PCS-100-022

Microvascular endothelial cells

• Primary Dermal Microvascular Endothelial Cells; Normal, Human, Neonatal, ATCC PCS-110-010

Smooth muscle cells derived from large vessels

• Primary Aortic Smooth Muscle Cells; Normal, Human, ATCC PCS-100-012
• Primary Coronary Artery Smooth Muscle Cells; Normal, Human, ATCC PCS-100-021
• Primary Pulmonary Artery Smooth Muscle Cells; Normal, Human, ATCC PCS-100-023

Muscle cells derived from heart

• Primary Cardiomyocytes; Normal, Human, ATCC PCS-120-010

1. For endothelial cells derived from large vessels (e.g., Normal Primary Human Umbilical Vein Endothelial Cells (HUVEC), ATCC PCS-100-010, Primary Umbilical Vein Endothelial Cells; Normal, Human, Pooled, ATCC PCS-100-013, Primary Aortic Endothelial Cells, ATCC PCS-100-011), supplement Vascular Cell Basal Medium with one of the following kits:
   1. Endothelial Cell Growth Kit-BBE (ATCC PCS-100-040)
   2. Endothelial Cell Growth Kit-VEGF (ATCC PCS-100-041)
2. For microvascular endothelial cells derived from normal human tissue (e.g., Primary Dermal Microvascular Endothelial Cells; Normal, Human, Neonatal, ATCC PCS-110-010), supplement Vascular Cell Basal Medium with one of the following kits:
   1. Microvascular Endothelial Cell Growth Kit-BBE (ATCC PCS-110-040)
   2. Microvascular Endothelial Cell Growth Kit-VEGF (ATCC PCS-110-041)
3. For smooth muscle cells derived from large vessels (e.g., Primary Aortic Smooth Muscle Cells, ATCC PCS-100-012), supplement Vascular Cell Basal Medium with the Vascular Smooth Muscle Cell Growth Kit (ATCC PCS-100-042).
4. Optional media supplements:
   1. Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
   2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
   3. Phenol Red (ATCC PCS-999-001)

1. Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
2. Thaw the components of the growth kit just prior to adding them to the basal medium. It is necessary to warm the         L-glutamine component in a 37°C water bath and shake to dissolve any precipitates prior to adding to the basal medium.
3. Obtain one bottle of Vascular Cell Basal Medium (475 mL) from cold storage.
4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
5. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the volume of each growth kit component, as indicated in Table 1, 2, 3, 4, or 5 to the bottle of basal medium using a separate sterile pipette for each transfer.

Table 1. If using the Endothelial Cell Growth Kit-BBE (ATCC^® PCS-100-040), add the indicated volume for each component: 

        Table 2. If using the Endothelial Cell Growth Kit-VEGF (ATCC^® PCS-100-041), add the indicated volume for each component:

       
Table 3. If using the Vascular Smooth Muscle Growth Kit (ATCC^® PCS-100-042), add the indicated volume for each component:

Table 4. If using the Microvascular Endothelial Cell Growth Kit-BBE (ATCC^® PCS-110-040), add the indicated volume for each component: 

       Table 5. If using the Microvascular Endothelial Cell Growth Kit-VEGF (ATCC^® PCS-110-041), add the indicated volume for each component:

Table 6. If using the Cardiomyocyte Growth Kit (ATCC^® PCS-120-040), add the indicated volume for each component:

Antimicrobials and phenol red are not required for proliferation but may be added if desired. The recommended volume of each optional component to be added to the complete growth media is summarized in Table 7.

Table 7. Addition of Antimicrobials/Antimycotics and Phenol Red (Optional)

6. Tightly cap the bottle of complete growth medium and swirl the contents gently to assure a homogeneous solution. Do not shake forcefully to avoid foaming. Label and date the bottle.
7. Complete growth media should be stored in the dark at 2°C to 8°C (do not freeze). When stored under these conditions, complete media is stable for 30 days. 

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

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