MYA-4890

Permits and Restrictions	View Permits
Strain Designations	S-1
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format	frozen
Type Strain	no

Preceptrol®	no
Comments	Plant pathogen (boxwood blight; causing a leaf spot disease on Pachysandra terminalis)
Morphology	On PDA medium at 25°C after 9 days, mycelium has concentric color zonation from reddish brown to buff with patches of dense white cottony mycelium. Produces a tan to dark reddish brown exudate. Hyphae guttulate, hyaline to reddish brown.

Medium	ATCC^® Medium 200: YM agar or YM broth ATCC^® Medium 336: Potato dextrose agar (PDA)
Growth Conditions	Temperature: 20°C to 25°C Atmosphere: Typical Aerobic

Sequenced Data	>18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence; GenBank number JX174049: GGGATCATTACCGAGTTTACAACTCCCAAACCCCATGTGAACATACCTGTTTCGTTCCCTCGGCGGTGTCCGGAAACGGCCCGCCAGAGGACCCAACAAACTCTTTTGAATTTATAGTATCTTCTGAGTGAAAAAAACAATAAATCAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCACCTTCGGGAGCTTGGTGTTGGGGATCGGCAGAGCGTCCTCCGGGTCGCGCCGTCCCCCAAATTTAGTGGCGGTCTCGCTGTAGCTTCCTCTGCGTAGTAATACACCTCGCTCTGGAGTCTCGGCGCGGCCACGCCGTAAAACCCCAACTTTTTCTGGTTGACCTCGAATCAGGTAGGACTACCCGCTGAACTTAAG >D1D2 region of the 28S ribosomal RNA gene; GenBank number JX174038: ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCTAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCCCTCGGGTCCGAGTTGTAATTTGTAGAGGATGCTTTTGGTGAGGTGCCTTCCGAGTTCCCTGGAACGGGACGCCATAGAGGGTGAGAGCCCCGTCTGGTTGGATACCGAGCCTCTGTAAAGCTCCTTCGACGAGTCGAGTAGTTTGGGAATGCTGCTCTAAATGGGAGGTATATGTCTTCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAAAAGTACGTGAAATTGTTGAAAGGGAAGCGCTTATGACCAGACTTGGGCTTGGTTGATCATCCTGGGTTCTCCCTGGTGCACTCTTCCAGTTCAGGCCAGCATCAGTTCGCTGCGGGGGATAAAAGCTTTGGGAATGTGGCTCCCTCGGGAGTGTTATAGCCCATTGCATAATACCCTGCTCCGGACTGAGGTTCGCGCATCTGCAAGGATGCTGGCGTAATGGTCATCAGCGA
Morphology	On PDA medium at 25°C after 9 days, mycelium has concentric color zonation from reddish brown to buff with patches of dense white cottony mycelium. Produces a tan to dark reddish brown exudate. Hyphae guttulate, hyaline to reddish brown.

Sequenced Data

>18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence; GenBank number JX174049:

GGGATCATTACCGAGTTTACAACTCCCAAACCCCATGTGAACATACCTGTTTCGTTCCCTCGGCGGTGTCCGGAAACGGCCCGCCAGAGGACCCAACAAACTCTTTTGAATTTATAGTATCTTCTGAGTGAAAAAAACAATAAATCAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCACCTTCGGGAGCTTGGTGTTGGGGATCGGCAGAGCGTCCTCCGGGTCGCGCCGTCCCCCAAATTTAGTGGCGGTCTCGCTGTAGCTTCCTCTGCGTAGTAATACACCTCGCTCTGGAGTCTCGGCGCGGCCACGCCGTAAAACCCCAACTTTTTCTGGTTGACCTCGAATCAGGTAGGACTACCCGCTGAACTTAAG

>D1D2 region of the 28S ribosomal RNA gene; GenBank number JX174038:

ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCTAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCCCTCGGGTCCGAGTTGTAATTTGTAGAGGATGCTTTTGGTGAGGTGCCTTCCGAGTTCCCTGGAACGGGACGCCATAGAGGGTGAGAGCCCCGTCTGGTTGGATACCGAGCCTCTGTAAAGCTCCTTCGACGAGTCGAGTAGTTTGGGAATGCTGCTCTAAATGGGAGGTATATGTCTTCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAAAAGTACGTGAAATTGTTGAAAGGGAAGCGCTTATGACCAGACTTGGGCTTGGTTGATCATCCTGGGTTCTCCCTGGTGCACTCTTCCAGTTCAGGCCAGCATCAGTTCGCTGCGGGGGATAAAAGCTTTGGGAATGTGGCTCCCTCGGGAGTGTTATAGCCCATTGCATAATACCCTGCTCCGGACTGAGGTTCGCGCATCTGCAAGGATGCTGGCGTAATGGTCATCAGCGA

Morphology

On PDA medium at 25°C after 9 days, mycelium has concentric color zonation from reddish brown to buff with patches of dense white cottony mycelium. Produces a tan to dark reddish brown exudate. Hyphae guttulate, hyaline to reddish brown.

Name of Depositor	JA LaMondia
Special Collection	ATCC
Chain of Custody	ATCC <-- J LaMondia
Isolation	Buxus sempervirens 'suffructicosa', Connecicut, USA.
Geographical Isolation	Connecticut, USA
Cross References	Nucleotide (GenBank) : JX174049 ITS including 5.8S rRNA gene Nucleotide (GenBank) : JX174038 D1/D2 region of 28S rRNA gene
References	LaMondia JA, et al. First report of Cylindrocladium pseudonaviculatum causing leaf spot of Pachysandra terminalis. Plant Dis. (in press).

Permits	These permits may be required for shipping this product: USDA APHIS PPQ Permit 526, plant pathogens permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. Isolation information for this item is required by USDA. Please check our website or contact Technical Service for assistance. Domestic isolates are easier to obtain than foreign isolates. Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis SDS

Permits

These permits may be required for shipping this product:

USDA APHIS PPQ Permit 526, plant pathogens permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. Isolation information for this item is required by USDA. Please check our website or contact Technical Service for assistance. Domestic isolates are easier to obtain than foreign isolates.
Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

Basic Documentation