SW 1783 [SW-1783, SW1783] (ATCC® HTB-13)

Organism: Homo sapiens, human  /  Tissue: brain  /  Disease: grade III,astrocytoma

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Organism Homo sapiens, human
Tissue
brain
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease grade III,astrocytoma
Age 68 years
Gender male
Ethnicity Caucasian
Karyotype hypertetraploid; modal number = 96. The rate of higher ploidies was 10.1%. The markers der(5)t(1;5) (p11;p15), t(1q3q), del(3) (q21), der(6)t(6;11) (p12;p15), der(11)t(6;11) (p12;p15), and 5 others were common to most cells. Of these, der (6) and der(11) had consistently two copies per cell, and were probably formed by balanced translocation between N6 and N11. Nine to 10 marker chromosomes were common to some cells, and 10 to 15 others were detected only once. All normal chromosomes were present in 2 to 5 copies per cell. Generally X had 3 copies and Y had 2 copies per cell. Double minutes (DM) were seen in some cells, and only 1 to a few copies occurred per cell.
Derivation The SW 1783 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1977. The cell line was received at the ATCC in January, 1982 at passage 15.
Clinical Data
68 years adult
Caucasian
male
Complete Growth Medium The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Interval: every 6 to 8 days
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Culture Conditions
Atmosphere: air, 100%
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,13
D13S317: 9,11
D16S539: 12
D5S818: 12,13
D7S820: 9,13
THO1: 6,8
TPOX: 8,11
vWA: 16,17
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
PGM1, 1
PGM3, 1
Name of Depositor A Leibovitz
Year of Origin 1977
References

Koochekpour S, et al. Met and hepatocyte growth factor/scatter factor expression in human gliomas. Cancer Res. 57: 5391-5398, 1997. PubMed: 9393765

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Koochekpour S, et al. Met and hepatocyte growth factor/scatter factor expression in human gliomas. Cancer Res. 57: 5391-5398, 1997. PubMed: 9393765