UC10-4F10-11 (ATCC® HB-304)

Organism: hamster (B cell); mouse (myeloma)  /  Cell Type:: hybridoma: B lymphocyte  / 

Permits and Restrictions

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Organism hamster (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The UC10-4F10-11 hybridoma cell line was produced by fusing Sp2/0-Ag14 myeloma cells with spleen cells from an Armenian hamster that had been immunized with the extracellular portion of of murine CTLA-4 fused to a murine IgG2a.
The antibody can be used to detect and quantitate CTLA-4 expression by flow cytometric analysis, and can be used to immunoprecipitate the antigen from CTLA-4 positive cells.
Under conditions of suboptimal T cell receptor (TCR) stimulation, whole antibody or Fab fragments from 4F10 can augment T cell proliferation in allogeneic mixed lymphocyte reactions.
However, when optimal TCR stimulation is present, 4F10 can suppress T cell proliferation.
Derivation
The UC10-4F10-11 hybridoma cell line was produced by fusing Sp2/0-Ag14 myeloma cells with spleen cells from an Armenian hamster that had been immunized with the extracellular portion of of murine CTLA-4 fused to a murine IgG2a.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse CTLA-4
Cellular Products
immunoglobulin; monoclonal antibody; against mouse CTLA-4
Comments
The UC10-4F10-11 hybridoma cell line was produced by fusing Sp2/0-Ag14 myeloma cells with spleen cells from an Armenian hamster that had been immunized with the extracellular portion of of murine CTLA-4 fused to a murine IgG2a.
The antibody can be used to detect and quantitate CTLA-4 expression by flow cytometric analysis, and can be used to immunoprecipitate the antigen from CTLA-4 positive cells.
Under conditions of suboptimal T cell receptor (TCR) stimulation, whole antibody or Fab fragments from 4F10 can augment T cell proliferation in allogeneic mixed lymphocyte reactions.
However, when optimal TCR stimulation is present, 4F10 can suppress T cell proliferation.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.01 mM nonessential amino acids and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG
Name of Depositor JA Bluestone
References

Walunas TL, et al. CTLA-4 can function as a negative regulator of T cell activation. Immunity 1: 405-413, 1994. PubMed: 7882171

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Walunas TL, et al. CTLA-4 can function as a negative regulator of T cell activation. Immunity 1: 405-413, 1994. PubMed: 7882171