44aacb (ATCC® HB-249)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Disease: Leukemia

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Leukemia
Applications
The antibody reacts with human CD11b, and blocks a variety of CD11b/CD18 mediated activities including neutrophil - substrate adherence and C3bi rosette formation on neutrophils, monocytes, macrophages and some NK cells.
The antibody cross-reacts with CD11b from non-human primates.
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human CD11b
Cellular Products
immunoglobulin; monoclonal antibody; against human CD11b
Comments
Animals were immunized with human acute monocytic leukemia cells (FAM M6).
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with human CD11b, and blocks a variety of CD11b/CD18 mediated activities including neutrophil - substrate adherence and C3bi rosette formation on neutrophils, monocytes, macrophages and some NK cells.
The antibody cross-reacts with CD11b from non-human primates.
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
  • heat-inactivated gamma globulin free horse serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere

Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG2a; kappa light chain
Name of Depositor RF Todd
References

Arnaout MA, et al. Inhibition of phagocytosis of complement C3- or immunoglobulin G-coated particles and of C3bi binding by monoclonal antibodies to a monocyte- granulocyte membrane glycoprotein (Mol). J. Clin. Invest. 72: 171-179, 1983. PubMed: 6874946

Simon RH, et al. Neutrophil-induced injury of rat pulmonary alveolar epithelial cells. J. Clin. Invest. 78: 1375-1386, 1986. PubMed: 3771800

Ismail G, et al. Prevention of pulmonary injury in isolated perfused rat lungs by activated human neutrophils preincubated with anti-Mo1 monoclonal antibody. Blood 69: 1167-1174, 1987. PubMed: 3030466

Dana N, et al. Two functional domains in the phagocyte membrane glycoprotein Mo1 identified with monoclonal antibodies. J. Immunol. 137: 3259-3263, 1986. PubMed: 2430017

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Arnaout MA, et al. Inhibition of phagocytosis of complement C3- or immunoglobulin G-coated particles and of C3bi binding by monoclonal antibodies to a monocyte- granulocyte membrane glycoprotein (Mol). J. Clin. Invest. 72: 171-179, 1983. PubMed: 6874946

Simon RH, et al. Neutrophil-induced injury of rat pulmonary alveolar epithelial cells. J. Clin. Invest. 78: 1375-1386, 1986. PubMed: 3771800

Ismail G, et al. Prevention of pulmonary injury in isolated perfused rat lungs by activated human neutrophils preincubated with anti-Mo1 monoclonal antibody. Blood 69: 1167-1174, 1987. PubMed: 3030466

Dana N, et al. Two functional domains in the phagocyte membrane glycoprotein Mo1 identified with monoclonal antibodies. J. Immunol. 137: 3259-3263, 1986. PubMed: 2430017