TS 106 Hybridoma; Mouse (HB-12497)

Designation / Description TS 106 Hybridoma; Mouse
U.S. Patent Number
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Animals were immunized with recombinant human thymidylate synthase (TS).
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
The antibody reacts specifically with the human protein TS and displays negligible cross-reactivity with other cellular proteins found in human cells.
The antibody may be used in immunohistochemical, Western Blot, and ELISA assays to detect TS in human colon cell lines.
Shipped frozen
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Johnson PG, et al. Monoclonal antibodies specific for human thymidylate synthase. US Patent 6,221,620 dated Apr 24 2001

Johnson PG, et al. Production and characterization of monoclonal antibodies that localize human thymidylate synthase in the cytoplasm of human cells and tissue. Cancer Res. 51: 6668-6676, 1992. PubMed: 1720706

Johnson PG, et al. Immunological quantitation of thymidylate synthase using the monoclonal antibody TS 106 in 5-fluorouracil-sensitive and -resistant human cancer cell lines. Cancer Res. 52: 4306-4312, 1992. PubMed: 1643628

Edler D, et al. Thymidylate synthase expression: an independent prognostic factor for local recurrence, distant metastasis, disease-free and overall survival in rectal cancer. Clin. Cancer Res. 6: 1378-1384, 2000. PubMed: 10778966

Edler D, et al. Immunohistochemical determination of thymidylate synthase in colorectal cancer--methodological studies. Eur. J. Cancer 33: 2278-2281, 1997. PubMed: 9470819

Edler D, et al. Immunohistochemically detected thymidylate synthase in colorectal cancer: an independent prognostic factor of survival. Clin. Cancer Res. 6: 488-492, 2000. PubMed: 10690528