Bacillus spizizenii (Nakamura et al.) Dunlap et al.
CRM-6633 ™
Download Genome Learn about the ATCC Genome Portal- Product category
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Bacteria
- Product type
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Certified reference material
- Classification
- Bacilli, Bacillales
- Strain designation
- NRS 231
- Applications
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Assay developmentBioinformatics
- Product format
- Freeze-dried
Documentation
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Detailed product information
General
- Specific applications
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For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. Assay of amoxicillinAssay of bekanamycin aminodeoxykanamycin, kanamycin B, kanendomycinAssay of capreomycin sulfate capreomycin, capromycinAssay of colistimethate sodiumAssay of colistin colimycinAssay of dactinomycin actinomycin, actinomycin DAssay of dihydrostreptomycin sulfate dihydrostreptomycinAssay of erythromycinAssay of framycetinBiotechnologyAssay of gentamicins gentamicinAssay of hygromycin BAssay of kanamycinAssay of lactam antibiotics beta-lactam antibioticsAssay of lasalocid X-537A, lasalocid AAssay of mitomycin mitomycin CAssay of monensinAssay of neomycinAssay of novobiocinAssay of penicillinAssay of polymyxin BAssay of rifampin rifampicin, rifamycin AMPAssay of spiramycinAssay of streptomycinAssay of streptonigrinAssay of tobramycinAssay of vancomycinControl strainMedia testingMembrane filter testingProduces restriction endonuclease Bsu6633IQuality control strainSporicidal testSterility testingSusceptibility disc testing novobiocinSusceptibility disc testing rifampin rifampicin, rifamycin AMPSusceptibility testingTesting antibacterial activityTesting bactericidesValidation control
- Preceptrol
- No
Characteristics
- Comments
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Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.
Certified Reference Material produced under an ISO 17034 accredited process.
CA Dunlap et al. reclassified Bacillus subtilis subsp. spizizenii into the new species Bacillus spizizenii. Some platforms and databases may still identify this species by its previous name.
Handling information
- Medium
- Handling procedure
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- Open vial according to enclosed instructions.
- From a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette. Rehydrate the entire pellet.
- Aseptically transfer this aliquot back into the broth tube. Mix well.
- Use several drops of the suspension to inoculate a second tube of broth, a slant, and/or a plate.
- Incubate all tubes and plates at 30°C for 24 hours.
- Handling notes
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Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.
Growth is poor in statically incubated broth, forming a thin pellicle leaving the broth clear. The colonial morphology of this strain varies considerably depending on the temperature of incubation, medium used, and length of time incubated. Colonies on Nutrient Agar may be dull and dry to shiny, irregularly-shaped, opaque, flat, erose, and irregular. As they age they appear to spread and become more uniform. Some colonies are adherent making them difficult to remove from the agar surface. On Tryptic Soy Agar the colonies are creamy, raised, erose, and mildly adherent with a soft sheen.
CA Dunlap et al. reclassified Bacillus subtilis subsp. spizizenii into the new species Bacillus spizizenii. Some platforms and databases may still identify this species by its previous name.
Additional information on this culture is available on the ATCC website at www.atcc.org.
Quality control specifications
- Verification method
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Whole-genome Sequencing
- Quality accreditation
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Manufactured under an ISO 17034 accredited process
History
- Deposited as
- Bacillus subtilis (Ehrenberg) Cohn
- Depositors
- NR Smith
- Special collection
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Certified Reference Materials
Legal disclaimers
- Intended use
- This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
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Certified Reference Material produced under an ISO 17034 accredited process.
- Warranty
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The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
- Disclaimers
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This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
Permits & Restrictions
Import Permit for the State of Hawaii
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Frequently Asked Questions
References
Curated Citations
Waleh NS, Ingraham JL. Pyrimidine ribonucleoside monophosphokinase and the mode of RNA turnover in Bacillus subtilis. Arch. Microbiol. 110: 49-54, 1976. PubMed: 189719
Shibata T, et al. Site-specific deoxyribonucleases in Bacillus subtilis and other Bacillus strains. J. Bacteriol. 128: 473-476, 1976. PubMed: 824277
Ikawa S, et al. Genetic studies on site-specific endodeoxyribonucleases in Bacillus subtilis: multiple modification and restriction systems in transformants of Bacillus subtilis 168. Mol. Gen. Genet. 177: 359-368, 1980. PubMed: 6246395
The genus Bacillus, Agriculture Handbook 427. Washington, DC: ARS/USDA; 1973.
British Pharmacopoeia Commission Biological assay of antibiotics. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XIV A, 2003
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