REC-1 (ATCC® CRL-3004)

Organism: Homo sapiens, human  /  Cell Type: lymphoblast  /  Disease: Mantle cell lymphoma (B cell non-Hodgkin's lymphoma)

Permits and Restrictions

View Permits

Organism Homo sapiens, human
Cell Type lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Mantle cell lymphoma (B cell non-Hodgkin's lymphoma)
Age 57 years
Gender male
Storage Conditions liquid nitrogen vapor phase
Karyotype This is a near-diploid cell line of male origin with a modal chromosome number of 45 and a polyploidy rate of 10%. The karyotype is highly rearranged with most of the cells containing the t(11;14)(q13;q32) common to mantle cell lymphoma. Also, there are approximately 5-6 derivative chromosomes in the karyology that have been described by others. Note: Cytogenetic information is based on initial seed stock at ATCC.
Images
Derivation
The human B-cell line Rec-1 was established from a t(11;14)(q13;q32) bearing lymphoma. RefRimokh R, et al. Detection of the chromosomal translocation t(11;14) by polymerase chain reaction in mantle cell lymphomas. Blood: 83(7):1871-1875, 1994. PubMed: 8142653
Clinical Data
male
Antigen Expression
CD3-, CD5-, CD10-, CD19+, CD20+, CD23-, FMC7- (verified at ATCC)
Oncogene p53
Genes Expressed
p53,CD3-, CD5-, CD10-, CD19+, CD20+, CD23-, FMC7- (verified at ATCC)
Comments
Cyclin D1 is overexpressed. RefSalaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365 
An ongoing level of chromosome rearrangements is observed. RefCamps, J., et al. Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697   
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 105 viable cells/mL. Maintain cultures at a cell concentration between 3 X 105 and 1 X 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL.

 

Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended.
Interval: Subculture when cell concentration is between 3x105 and 1x106 cells/mL.
Medium renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation
Freeze medium: fetal bovine serum, 60%; RPMI-1640 Medium, 30%; DMSO, 10%
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10
D16S539: 11
D5S818: 12,13
D7S820: 10,11
THO1: 9,9.3
TPOX: 8,9
vWA: 17
Population Doubling Time approximately 38 hours
Name of Depositor E. Campo
Year of Origin 2003
References

Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365

Camps, J., et al. Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697

Rimokh R, et al. Detection of the chromosomal translocation t(11;14) by polymerase chain reaction in mantle cell lymphomas. Blood: 83(7):1871-1875, 1994. PubMed: 8142653

Pinyol M, et al. Inactivation of RB1 in mantle-cell lymphoma detected by nonsense-mediated mRNA decay pathway inhibition and microarray analysis. Blood: 109(12):5422-5429, 2007. PubMed: 17332242

Tagawa H, et al. Genome-wide array-based CGH for mantle-cell lymphoma: identification of homozygous deletions of the proapoptotic gene BIM. Oncogene, 1-11, 2004

Drexler HG, MacLeod RA. Malignant hematopoietic cell lines: in vitro models for the study of mantle cell lymphoma. Leuk. Res. 26: 787-787, 2002. PubMed: 12127550

REC-1 Product Information, DSMZ (German Collection of Microorganisms and Cell Cultures). Retreived July 09, 2007, from http://www.dsmz.de/index.htm.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365

Camps, J., et al. Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697

Rimokh R, et al. Detection of the chromosomal translocation t(11;14) by polymerase chain reaction in mantle cell lymphomas. Blood: 83(7):1871-1875, 1994. PubMed: 8142653

Pinyol M, et al. Inactivation of RB1 in mantle-cell lymphoma detected by nonsense-mediated mRNA decay pathway inhibition and microarray analysis. Blood: 109(12):5422-5429, 2007. PubMed: 17332242

Tagawa H, et al. Genome-wide array-based CGH for mantle-cell lymphoma: identification of homozygous deletions of the proapoptotic gene BIM. Oncogene, 1-11, 2004

Drexler HG, MacLeod RA. Malignant hematopoietic cell lines: in vitro models for the study of mantle cell lymphoma. Leuk. Res. 26: 787-787, 2002. PubMed: 12127550

REC-1 Product Information, DSMZ (German Collection of Microorganisms and Cell Cultures). Retreived July 09, 2007, from http://www.dsmz.de/index.htm.