Z-138 (ATCC® CRL-3001)

Organism: Homo sapiens, human  /  Cell Type: lymphoblast  /  Tissue: Mantle cell lymphoma (B cell non-Hodgkin's lymphoma)  /  Disease: mantle cell lymphoma

Organism Homo sapiens, human
Tissue Mantle cell lymphoma (B cell non-Hodgkin's lymphoma)
Cell Type lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease mantle cell lymphoma
Age 70 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype This is a hyperdiploid human cell line of male origin with a modal chromosome number of 49 and a 4% polyploidy rate. Most of the cells examined contained the t(11;14)(q13;q32) derivative chromosome common to Mantle Cell Lymphoma. Other derivative chromosomes present in most of the examined cells included: del(5)(p15), der(9)t(9;?), der(14)t(8;14)?, and add(17p). Overall, the karyology was consistent with published data.
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Derivation
The Z-138 cell line was derived from a patient who initially was diagnosed with chronic lymphocytic leukemia (CLL). About 2 years later, the neoplasm had transformed into an aggressive, mature B-cell acute lymphocytic leukemia (B-cell ALL). According to the criteria of the current World Health Organization lymphoma classification, this neoplasm is best classified as mantle cell lymphoma, with blastoid transformation present in the terminal phase of disease.
Clinical Data
70 years
Caucasian
male
The Z-138 cell line was derived from a patient who initially was diagnosed with chronic lymphocytic leukemia (CLL). About 2 years later, the neoplasm had transformed into an aggressive, mature B-cell acute lymphocytic leukemia (B-cell ALL). According to the criteria of the current World Health Organization lymphoma classification, this neoplasm is best classified as mantle cell lymphoma, with blastoid transformation present in the terminal phase of disease.
Antigen Expression
CD3-, CD5-, CD10-, CD19+, CD20+, CD23+, FMC7- (verified at ATCC)
Genes Expressed
CD3-, CD5-, CD10-, CD19+, CD20+, CD23+, FMC7- (verified at ATCC)
Comments
Z-138 cells have a complex karyotype, including the t(11;14)(q13;q32) abnormality, which is common in B-cell neoplasms.
These cells overexpress Cyclin D1.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%
Subculturing

Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 to 2 x 105 viable cells/mL. Do not allow the cell concentration to exceed 3 x 106 cells/mL.  

Subcultivation ratio: A subcultivation ratio of 1:5 to 1:15 is recommended.
Medium renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation
Freeze medium: horse serum 90%; DMSO, 10%
liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 9,12
D16S539: 11
D5S818: 11,13
D7S820: 8
TPOX: 8
THO1: 6
vWA: 15,18
Population Doubling Time approximately 24 hours
Name of Depositor Z Estrov
Year of Origin August 1990
References

Estrov Z, et al . Z-138: a new mature B-cell acute lymphoblastic leukemia cell line from a patient with transformed chronic lymphocytic leukemia. Leuk. Res.:22 (4):341-353, 1998. PubMed 9669839

Medeiros LJ, et al. Z-138 cell line was derived from a patient with blastoid variant mantle cell lymphoma. Leuk. Res. 30(4):497-501, 2006. PubMed 16203034

Tucker CA, et al. Four human t(11;14)(q13;q32)-containing cell lines having classic and variant features of Mantle Cell Lymphoma. Leuk. Res. 30: 449-457, 2006. PubMed: 16183118

Basic Documentation
Other Documentation
References

Estrov Z, et al . Z-138: a new mature B-cell acute lymphoblastic leukemia cell line from a patient with transformed chronic lymphocytic leukemia. Leuk. Res.:22 (4):341-353, 1998. PubMed 9669839

Medeiros LJ, et al. Z-138 cell line was derived from a patient with blastoid variant mantle cell lymphoma. Leuk. Res. 30(4):497-501, 2006. PubMed 16203034

Tucker CA, et al. Four human t(11;14)(q13;q32)-containing cell lines having classic and variant features of Mantle Cell Lymphoma. Leuk. Res. 30: 449-457, 2006. PubMed: 16183118