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CON.1 (ATCC^® CRL-2229^™)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) / Cell Type:: hybridoma: B lymphocyte /

CON.1 ATCC^® CRL-2229^™

frozen

For-Profit: $551.00 Non-Profit: $459.17

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Permits and Restrictions	View Permits View Restrictions
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	Animals were immunized with a peptide corresponding to residues 23 to 44 in bovine clathrin LCa and LCb (a region that is conserved in all mammalian clathrin light chains). The antibody is useful for western blotting, immunofluorescence and immunoprecipitation applications involving clathrin light chains of mammals.

Derivation	Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed	immunoglobulin; monoclonal antibody; against human clathrin light chain
Cellular Products	immunoglobulin; monoclonal antibody; against human clathrin light chain
Comments	Animals were immunized with a peptide corresponding to residues 23 to 44 in bovine clathrin LCa and LCb (a region that is conserved in all mammalian clathrin light chains). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is useful for western blotting, immunofluorescence and immunoprecipitation applications involving clathrin light chains of mammals.

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 0.5 X 10 exp5 to 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation	Culture medium, 95%; DMSO, 5%

Isotype	IgG2b

Name of Depositor	FM Brodsky
References	Nathke IS, et al. Folding and trimerization of clathrin subunits at the triskelion hub. Cell 68: 899-910, 1992. PubMed: 1547490

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis SDS
Restrictions	Note: These cells are distributed for research purposes only subject to the following: 1) The CON.1 cell line or its products must not be distributed to third parties. Commercial interests are the exclusive property of Frances M. Brodsky and Becton Dickinson Immunocytometry Systems. 2) Any proposed commercial use of these cells or their products must first be negotiated with Frances M. Brodsky and Becton Dickinson Immunocytometry Systems. 3) In all papers reporting any use of these cells or derived products, a direct reference will be made to Cell 68:899-910, 1992
References	Nathke IS, et al. Folding and trimerization of clathrin subunits at the triskelion hub. Cell 68: 899-910, 1992. PubMed: 1547490