Derivation
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Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
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Genes Expressed
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immunoglobulin; monoclonal antibody; against human clathrin light chain
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Cellular Products
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immunoglobulin; monoclonal antibody; against human clathrin light chain
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Comments
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Animals were immunized with a peptide corresponding to residues 23 to 44 in bovine clathrin LCa and LCb (a region that is conserved in all mammalian clathrin light chains). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is useful for western blotting, immunofluorescence and immunoprecipitation applications involving clathrin light chains of mammals.
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