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FL (ATCC® CCL-62)

Organism: Homo sapiens, human  / 

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Organism Homo sapiens, human
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Gender female
Applications
This line was originally thought to be derived from normal amnion, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.
The cells are positive for keratin by immunoperoxidase staining.
NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination
Derivation
This line was originally thought to be derived from normal amnion, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.
Clinical Data
female
HeLa Markers Y
Genes Expressed
keratin,The cells are positive for keratin by immunoperoxidase staining.
Cellular Products
keratin
Comments
This line was originally thought to be derived from normal amnion, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.
The cells are positive for keratin by immunoperoxidase staining.
NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  • Remove and discard culture medium.
  • Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  • Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
  • Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting
  • Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
  • Incubate cultures at 37C.
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 9,10
D13S317: 12,13.3
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
TH01: 7
TPOX: 8,12
vWA: 16,18
Isoenzymes
G6PD, A
Name of Depositor J Fogh
Deposited As Homo sapiens
References

Fogh J, Lund RO. Continuous cultivation of epithelial cell strain (FL) from human amniotic membrane. Proc. Soc. Exp. Biol. Med. 94: 532-537, 1957. PubMed: 13408317

Fogh J, et al. Sensitivity of FL cells to polio- and adenoviruses. Arch. Gesamte Virusforsch. 9: 559-570, 1960. PubMed: 13823673

Cancer Res. 18: 692, 1958.

Fogh J, Edwards GA. Ultrastructure of primary culture amnion cells and transformed FL cells in continuous culture. J. Natl. Cancer Inst. 23: 893-923, 1959. PubMed: 13823672

. . Proc. Soc. Exp. Biol. Med. 119: 223, 1965.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Fogh J, Lund RO. Continuous cultivation of epithelial cell strain (FL) from human amniotic membrane. Proc. Soc. Exp. Biol. Med. 94: 532-537, 1957. PubMed: 13408317

Fogh J, et al. Sensitivity of FL cells to polio- and adenoviruses. Arch. Gesamte Virusforsch. 9: 559-570, 1960. PubMed: 13823673

Cancer Res. 18: 692, 1958.

Fogh J, Edwards GA. Ultrastructure of primary culture amnion cells and transformed FL cells in continuous culture. J. Natl. Cancer Inst. 23: 893-923, 1959. PubMed: 13823672

. . Proc. Soc. Exp. Biol. Med. 119: 223, 1965.