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Escherichia coli (Migula) Castellani and Chalmers (ATCC® BAA-3053)

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Strain Designations: 1013491  /  Type Strain: no  /  Biosafety Level: 2

Permits and Restrictions

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Reports Upon your purchase of this product, you will receive a report containing MIC values, a susceptibility profile, and the DNA sequence information for predicted annotated antibiotic resistance genes and 16S rRNA genes. This report will be provided in multiple formats (.pdf, .txt, .xls, .zip).
Deposited As Escherichia coli
Verified By Whole-genome Sequencing
Classification Enterobacteriaceae, Escherichia
Strain Designations 1013491
Application Drug discovery and development
Development of molecular-based detection assays
Development of updated sterility protocols
Isolation Urine
Isolation Date 2013
Geographic Source Greece
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -80°C or colder
Preceptrol® no
Annotated Genes AAC(3)-IId
AAC(6')-Ib (Partial)
aadA2
acrA
acrB
acrD
acrE
acrF
acrR
AcrS
ampC
ampH
bacA
baeR
baeS
catI
cpxA
CRP
dfrA12
EF-Tu
emrA
emrB
emrK
emrR
emrY (Partial)
eptA
evgA
evgS
gadW
gadX
GlpT
gyrA
H-NS
kdpE
marA
marR (Partial)
mdfA
mdtA
mdtB
mdtC
mdtE
mdtF
mdtG
mdtH
mdtI
mdtJ
MdtK
mdtM
mdtN
mdtO
mdtP
mphA
mphB
msbA
ompN
parC
PBP4B
PmrF
QacEDelta
rosA
rosB
sdiA
soxR
soxS
sul1
TEM-1
tolC
ugd
YojI
Type Strain no
MIC Range Resistant
Ciprofloxacin: ≥4 µg/mL
Levofloxacin: ≥8 µg/mL


Susceptible
Amikacin: ≤16 µg/mL
Aztreonam: ≤4 µg/mL
Cefepime: ≤2 µg/mL
Ceftazidime: ≤4 µg/mL
Ceftazidime-avibactam: ≤8 µg/mL
Ceftriaxone: ≤1 µg/mL
Colistin: ≤2 µg/mL
Colistin-P80: ≤2 µg/mL
Doripenem: ≤1 µg/mL
Imipenem: ≤1 µg/mL
Meropenem: ≤1 µg/mL
Piperacillin-tazobactam: ≤16/4 µg/mL


Comments This strain is part of the Global Priority Superbugs collection. It is an extensively characterized antimicrobial-resistant clinical isolate with validated genotypic and phenotypic activity against a variety of drug classes. This strain is provided with geographic source information; an NGS-assembled whole genome sequence, including annotated antibiotic resistance and rRNA genes; and an expanded level of susceptibility data with MIC test results.

Minimum inhibitory concentration (MIC) ranges for resistant, intermediate, and susceptible are based on criteria within the Clinical and Laboratory Standards Institute (CLSI) Performance Standards for Antimicrobial Susceptibility Testing, 30th Edition.

Predicted antimicrobial resistance gene sequence data are provided for informational purposes only and are based on open reading frames (ORFs) identified and annotated from contigs produced during next-generation sequencing (NGS). ATCC does not warrant that the entire predicted antimicrobial resistance gene is present within the sequence prepared for lot-specific reports. Likewise, the information provided merely indicates that some portion of the sequence (whole or in part) is present based on analytical base pair alignment with known gene sequences.
Medium ATCC® Medium 18: Trypticase Soy Agar/Broth
Growth Conditions Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor International Health Management Associates (IHMA), Inc.
Special Collection Global Priority Superbugs
Chain of Custody ATCC <-- International Health Management Associates (IHMA), Inc.
Isolation Urine
Isolation Date 2013
Geographic Source Greece
Patient Age 54
Patient Gender Female
References

Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing, 30th Edition. Wayne, PA. M100, 2020.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • The Clinical Isolates Collection (CIC) products are distributed under the additional terms of the Addendum for CIC Materials. It is required that the Addendum be signed and returned to ATCC prior to shipment.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions
Restrictions

Products in the Global Priority Superbugs collection are distributed under the additional terms of the Addendum for Global Priority Superbugs Materials. It is required that the Addendum be signed and returned to ATCC prior to shipment.

FAQs
  1. Accessing genome sequencing data


    Date Updated: 9/30/2019

  2. Criteria for reference quality genomes


    Date Updated: 9/30/2019