GCH1 (ATCC® 87665)

Organism: Cryptosporidium parvum Tyzzer  /  Clone Type: Library  /  Depositors: SJ Upton, NV Khramtsov

Permits and Restrictions
Permits and Restrictions

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Designations GCH1
Species Cryptosporidium parvum Tyzzer
Depositors SJ Upton, NV Khramtsov
Origin
sporozoites/oocysts
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II SK+
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
Cross references:
Vector
Tissue: sporozoites/oocysts
DNA: genomic
Digest: Complete
Insert size range: 0.2 - 2.5kb
Coverage: Clones for "complete" coverage: 20000.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: frozen
Comments
DNA was isolated from oocysts of Crytosporidium parvum, GCH1 isolate, obtained from the feces of an AIDS patient by Dr. Jeff Griffiths at Tufts University in 1991. Since that time, oocysts have been propagated in experimentally infected calves.
References

Khramtsov NV, et al. Cloning and analysis of a Cryptosporidium parvum gene encoding a protein with homology to cytoplasmic form hsp70. J. Eukaryot. Microbiol. 42: 416-422, 1995. PubMed: 7620467

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