pCM190 (ATCC® 87662)

Applications: YE-type (episomal) shuttle vectorexpression vector  /  Depositors: E Herrero

Permits and Restrictions

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Designations pCM190
Depositors E Herrero
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 8.0120000839233400
Vector: pCM190 (plasmid)
Promoters: Promoter CMV
Marker(s):URA3,ampR
Construct size (kb): 8.01200008392334
Features: marker(s): URA3
marker(s): ampR
operator: tetO - seven repeats
other: tTA transcriptional activator
promoter: CMV
promoter: CYC1
replicon: 2 micron
replicon: pMB1
MCS: BamHI...PstI
terminator: ADH1
terminator: CYC1
Applications
YE-type (episomal) shuttle vector
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--8.0; PstI--8.0; BamHI/XhoI--7.2, 0.7.
Yeast episomal expression vector driven by the tetO-CYC1 promoter. One of 7 tetracycline-regulated expression vectors (ATCC 87656 - 87662) differing in the level of expression, mode of replication and selectable marker.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Gari E, et al. A set of vectors with a tetracycline-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiae. Yeast 13: 837-848, 1997. PubMed: 9234672

Related Products
component of:ATCC 87663
Shipped frozen
Shipping Information

 Frozen glycerol stock of Escherichia coli DH5alpha containing the plasmid.

Product Sheet
Product Sheet
Product Sheet
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