YEpFAT7 (ATCC® 87645)

Applications: Selection for URA3 expression produces a plasmid copy number of 20-40; growth on YC minus leucine produces a copy number of 100-200.  /  Depositors: K Runge

Permits and Restrictions

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Designations YEpFAT7
Depositors K Runge
Biosafety Level 1
Host
Distribution host: Escherichia coli MC1066
Vector Information
Size (kb): 10.3179998397827200
DESCRIPTION OF VECTOR:
Intact vector size: 10.318
Type of vector: plasmid
Cloning sites: SacI SmaI SalI
Polylinker sites: EcoRI SacI AvaI KpnI SmaI BamHI XbaI SalI PstI HindIII
Other unique sites: NotI
Host range: Schizosaccharomyces pombe; Saccharomyces cerevisiae;
Escherichia coli
Features (with orientation and position when available):
promoter for in vitro transcription: T7, ->, 9016-9035
MCS: EcoRI...HindIII, ->, 10269-2
marker(s): GART, <-, 2-2091
replicon: 2 micron/LEU2-d, 2086-4780
marker(s): LEU2-d, ->, 3443-4537
marker(s): URA3, <-, 4860-5662
promoter for in vitro transcription: T3, <-, 5895-5914
promoter: lac, <-, 5984-6012
replicon: pMB1, 6338-6338
marker(s): ampR, <-, 7096-7956
Vector: YEpFAT7 (plasmid)
Promoters: Promoter for in vitro transcription T7
Marker(s):URA3,ampR,LEU2-d
Construct size (kb): 10.31799983978272
Features: marker(s): GART
marker(s): LEU2-d
marker(s): URA3
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron/LEU2-d
replicon: pMB1
MCS: EcoRI...HindIII
Applications
Selection for URA3 expression produces a plasmid copy number of 20-40; growth on YC minus leucine produces a copy number of 100-200.
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--9.6, 1.0; SmaI--11.0; HindIII--6.2, 4.8.
Selection for URA3 expression produces a plasmid copy number of 20-40; growth on YC minus leucine produces a copy number of 100-200.
One of 9 YE-type shuttle vectors (ATCC 87541, 87554, 87560 an d 87640 - 87645) that have the leu2-d gene, which is correlated with high copy number in S. cerevisiae, when grown in medium lacking leucine.
Media M9 + tryptophan + leucine
Growth Conditions
Temperature: 37.0°C
References

Runge KW, Zakian VA. Introduction of extra telomeric DNA sequences into Saccharomyces cerevisiae results in telomere elongation. Mol. Cell. Biol. 9: 1488-1497, 1989. PubMed: 2657397

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet
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