Distribution host: Escherichia coli
HB101 (ATCC 33694)
Size (kb): 4.6430001258850100
DESCRIPTION OF VECTOR:
Intact vector size: 4.643
Type of vector: plasmid
Cloning sites: HindIII PstI SalI BamHI ClaI SmaI KpnI SacI EcoRI
Polylinker sites: HindIII PstI SalI BamHI [tk-promoter luc+ SV40 poly (A)]ClaI
SmaI KpnI SacI EcoRI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
MCS: HindIII...BamHI, ->, 1-36
promoter: truncated TK promoter, 37-131
reporter group: luciferase (luc+), ->, 170-1822
terminator: SV40 late polyadenylation, 1854-2075
MCS: ClaI...EcoRI, 2078-2106
replicon: pMB1, 2519-2519
marker(s): ampR, <-, 3277-4137
terminator: SV40 large T-antigen polyadenylation, 4269-4640
Vector: pTATALUC+ (plasmid)
Promoters: Promoter truncated TK promoter
Construct size (kb): 4.64300012588501
Features: marker(s): ampR
promoter: truncated TK promoter
reporter group: luciferase (luc+)
terminator: SV40 large T-antigen polyadenylation
terminator: SV40 late polyadenylation
Restriction digests of the clone give the following sizes (kb): BamHI--4.6; HindIII--4.6; EcoRI--4.6.
The vector contains modified luciferase (luc+) gene from the firefly Photinus pyralis in order to increase the yield of recoverable luciferase activity after transfection and to eliminate potential cryptic regulatory elements.
The promoters driving luc+ transcription in these two vectors represent different 5' deletions of the tk promoter ending at -32 and -105, respectively.
The vector pTATALUC+ carries only the TATA box to avoid interference of the tk-derived CAAT and GC boxes with the regulatory elements of interest.
The optimized luciferase reporter gene vectors (ATCC 87630
- 87633) provide valuable tools for the analysis of eukaryotic regulatory DNA elements.
The vectors pTATALUC+ (ATCC 87631)
and ptkLUC+ (ATCC 87632)
were designed for the characterization of isolated cis regulatory elements.
Altschmied J, Duschl J. Set of optimized luciferase reporter gene plasmids compatible with widely used CAT vectors. BioTechniques 23: 436-438, 1997. PubMed: 9298214