pTATALUC+ (ATCC® 87631)

Applications: reporter constructshuttle vector  /  Depositors: J Duschl, J Altschmied

Permits and Restrictions

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Designations pTATALUC+
Depositors J Duschl, J Altschmied
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 4.6430001258850100
DESCRIPTION OF VECTOR:
Intact vector size: 4.643
Type of vector: plasmid
Cloning sites: HindIII PstI SalI BamHI ClaI SmaI KpnI SacI EcoRI
Polylinker sites: HindIII PstI SalI BamHI [tk-promoter luc+ SV40 poly (A)]ClaI
SmaI KpnI SacI EcoRI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
MCS: HindIII...BamHI, ->, 1-36
promoter: truncated TK promoter, 37-131
reporter group: luciferase (luc+), ->, 170-1822
terminator: SV40 late polyadenylation, 1854-2075
MCS: ClaI...EcoRI, 2078-2106
replicon: pMB1, 2519-2519
marker(s): ampR, <-, 3277-4137
terminator: SV40 large T-antigen polyadenylation, 4269-4640
Vector: pTATALUC+ (plasmid)
Promoters: Promoter truncated TK promoter
Marker(s):ampR
Construct size (kb): 4.64300012588501
Features: marker(s): ampR
promoter: truncated TK promoter
replicon: pMB1
reporter group: luciferase (luc+)
MCS: ClaI...EcoRI
MCS: HindIII...BamHI
terminator: SV40 large T-antigen polyadenylation
terminator: SV40 late polyadenylation
Applications
reporter construct
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.6; HindIII--4.6; EcoRI--4.6.
The vector contains modified luciferase (luc+) gene from the firefly Photinus pyralis in order to increase the yield of recoverable luciferase activity after transfection and to eliminate potential cryptic regulatory elements.
The promoters driving luc+ transcription in these two vectors represent different 5' deletions of the tk promoter ending at -32 and -105, respectively.
The vector pTATALUC+ carries only the TATA box to avoid interference of the tk-derived CAAT and GC boxes with the regulatory elements of interest.
The optimized luciferase reporter gene vectors (ATCC 87630 - 87633) provide valuable tools for the analysis of eukaryotic regulatory DNA elements.
The vectors pTATALUC+ (ATCC 87631) and ptkLUC+ (ATCC 87632) were designed for the characterization of isolated cis regulatory elements.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Altschmied J, Duschl J. Set of optimized luciferase reporter gene plasmids compatible with widely used CAT vectors. BioTechniques 23: 436-438, 1997. PubMed: 9298214

Related Products
component of:ATCC 87654
Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Restrictions This product's use is governed by a Label License. For information on purchasing a license to use this product for purposes other than those permitted in the Label License, please contact Promega.
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