pRS413 (ATCC® 87518)

Applications: YC-type (centromeric) shuttle vector  /  Depositors: P Hieter

Designations pRS413
Permits and Restrictions

View Permits

Depositors P Hieter
Biosafety Level 1
Distribution host: Escherichia coli DH5alpha
Vector Information
Size (kb): 4.97
Intact vector size: 4.970
Type of vector: phagemid
Cloning sites: ApaI XhoI SalI ClaI EcoRV EcoRI SmaI BamHI SpeI XbaI EagI
NotI SacII SacI NaeI
Polylinker sites: BssHII KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI
SmaI BamHI SpeI XbaI EagI NotI BstXI SacII SacI BssHII
Construction: pBluescriptII KS+
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): HIS3, ->, 504-1163
replicon: f1, <-, 1473-1879
insert detection: lacZ', <-, 1880-2217
promoter for in vitro transcription: T7, ->, 2049-2068
MCS: KpnI...SacI, ->, 2076-2183
promoter for in vitro transcription: T3, <-, 2195-2214
promoter: lac, <-, 2283-2312
replicon: pMB1, 2638-2638
marker(s): ampR, <-, 3396-4256
centromere: CEN6, 4398-4514
replicon: ARSH4, 4512-4885
Vector: pRS413 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pBluescriptII KS+
Construct size (kb): 4.97
Features: insert detection: lacZ'
marker(s): HIS3
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: ARSH4
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
centromere: CEN6
YC-type (centromeric) shuttle vector
Restriction digests of the clone give the following sizes (kb): PvuII--4.55, 0.45; BglI--2.3, 1.7, 1.0; PstI--4.05, 0.95.
A YC-type shuttle vector with HIS3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436

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component of:ATCC 87531
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