pRS411 (ATCC® 87474)

Applications: YC-type (centromeric) shuttle vector  vector permitting visual detection of recombinants O-acetylhomoserine sulfhydrylase  /  Depositors: JD Boeke

Designations pRS411
Permits and Restrictions

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Depositors JD Boeke
Biosafety Level 1
Host Escherichia coli HB101 (ATCC® 33694™)
Vector Information
Intact vector size: 5.407
Type of vector: phagemid
Cloning sites: SacI BstXI SacII EagI NotI SpeI BamHI SmaI PstI ClaI SalI XhoI ApaI KpnI
Polylinker sites: SacI BstXI SacII EagI NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV ClaI SalI XhoI ApaI KpnI
Construction: pRS401, pRS84
Host range: Saccharomyces cerevisiae; Escherichia coli

Features (with orientation and position when available):
marker(s): MET15, →, 421-1270
replicon: f1, ←, 1860-2316
promoter for in vitro transcription: T7, →, 2486-2505
MCS: KpnI...SacI, →, 2513-2615
promoter for in vitro transcription: T3, ←, 2632-2651
coding sequence: lacZ', ←, 2317-2676
promoter: lac, ←, 2721-2749
replicon: pMB1, 3075-3075
marker(s): ampR, ←, 3833-4693
centromere: CEN6, 4835-4951
replicon: ARS4, 4952-5353
Vector: pRS411 (phagemid)
Applications
YC-type (centromeric) shuttle vector 
vector permitting visual detection of recombinants O-acetylhomoserine sulfhydrylase
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.4; EcoRI--3.7, 1.7; XbaI--3.4, 2.0.
met15 phenotype produces brown colonies when grown on Pb containing media.
MET15, MET17 and MET25 are synonymous.
This vector is useful for gene knockout experiments in hosts with a non-revertable met15 auxotrophic marker gene mutation.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
References

Cost GJ, Boeke JD. A useful colony colour phenotype associated with the yeast selectable/counter-selectable marker MET15. Yeast 12: 939-941, 1996. PubMed: 8873447

Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801

Related Products
Component of: ATCC 87476
Component of: ATCC 87529
Shipped frozen
Shipping Information Plasmid in E. coli