p425 GALL (ATCC® 87339)

Applications: YX-type (expression) shuttle vector  /  Depositors: M Funk

Permits and Restrictions

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Designations p425 GALL
Depositors M Funk
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 7.5100002288818360
DESCRIPTION OF VECTOR:
Intact vector size: 7.510
Type of vector: plasmid
Cloning sites: SpeI BamHI SmaI PstI HindIII SalI XhoI
Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI
Construction: pRS425, GALL promoter
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): LEU2, ->
terminator: CYC1, ->
MCS: SpeI...XhoI, <-
promoter for expression: GALL, <-
marker(s): ampR, <-
replicon: 2 micron, ->
Vector: p425 GALL (plasmid)
Promoters: Promoter for expression GALL
Construction: pRS425, GALL promoter
Marker(s):LEU2,ampR
Construct size (kb): 7.510000228881836
Features: marker(s): LEU2
marker(s): ampR
promoter for expression: GALL
replicon: 2 micron
MCS: SpeI...XhoI
terminator: CYC1
Applications
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--7.8; EcoRI--5.4, 2.3; SacI/XbaI--3.9, 3.2.
The galactokinase (GALL) promoter is a deletion variant of GAL1, which lacks one of the 3 UAS elements required for full induction of the promoter by galactose.
The promoter is tightly repressed by glucose and is induced at a moderate level by galactose.
High copy number shuttle expression vector.
One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736

Related Products
component of:ATCC 87670
Shipped frozen
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 87339