lambdaACT2 - human prostate cDNA (ATCC® 87296)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: SJ Elledge

Permits and Restrictions

View Permits

Designations lambdaACT2 - human prostate cDNA
Species Homo sapiens, human
Depositors SJ Elledge
Origin
prostate
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdaACTII
Intact vector size: 42.300
Type of vector: phage
Vector end: EcoRI
Vector end: XhoI
Cloning sites: NcoI SfiI SmaI BamHI EcoRI XhoI BglII
Polylinker sites: NdeI NcoI SfiI SmaI BamHI EcoRI XhoI BglII
Construction: lambdaYES, pACT2 (ATCC 87006)
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, LEU2
promoter: ADC1
replicon: pMB1, 2 micron, lambda
repressor gene: cI857
terminator: ADC1
Cross references:
Vector
Tissue: prostate
DNA: cDNA
Insert size range: 0.7 - 7 kb
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen bacteria-free lysate (volume: 0.4 mL)
Comments
In yeast, the ADH promoter drives expression of a fusion protein consisting of the GAL4 activation domain, a hemagglutinin tag, and the sequences directed by the insert.
References

Durfee T, et al. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7: 555-569, 1993. PubMed: 8384581

Stephen J Elledge, personal communication

Product Sheet
Product Sheet
Product Sheet