lambdaACT - Drosophila third instar cDNA (ATCC® 87290)

Organism: Drosophila melanogaster, fruit fly  /  Clone Type: Library  /  Depositors: SJ Elledge

Designations lambdaACT - Drosophila third instar cDNA
Species Drosophila melanogaster, fruit fly
Depositors SJ Elledge
larva, third instar
Name of vector: lambdaACT
Intact vector size: 42.400
Type of vector: phage
Vector end: XhoI
Vector end: XhoI
Cloning sites: XhoI
Polylinker sites: BglII EcoRI BamHI XhoI BglII
Construction: lambdaYES, pSE1107
Host range: Escherichia coli
Features (with orientation and position when available):
other: left arm (19.6 kb), ->
restriction site: NotI, ->
other: lox, ->
marker(s): LEU2, <-
replicon: 2 micron
marker(s): ampR, ->
replicon: pMB1
promoter: ADH, ->
other: GAL4 activation domain, ->
MCS: BglII/EcoRI...XhoI/BglII, ->
terminator: ADH, ->
other: lox, ->
restriction site: NotI
other: right arm (15.2 kb, with cI857)
Cross references:
Tissue: larva, third instar
Insert size range: 0.7 - 7.0 kb
Insert ends: XhoI adaptors
Insert site: XhoI
Number of independent recombinants: 1 x 10^8
Non-recombinants (%): 5
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen bacteria-free lysate (volume: 0.4 mL)

Durfee T, et al. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7: 555-569, 1993. PubMed: 8384581