lambdaTRP - Saccharomyces cerevisiae cDNA (ATCC® 87277)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen  /  Clone Type: Library  /  Depositors: SJ Elledge

Permits and Restrictions

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Designations lambdaTRP - Saccharomyces cerevisiae cDNA
Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors SJ Elledge
Name of vector: lambdaTRP
Intact vector size: 42.400
Type of vector: phage
Vector end: XhoI
Vector end: XhoI
Cloning sites: XhoI
Polylinker sites: EcoRI XhoI SacI
Cloning capacity: 0 - 8.0 kb
Construction: lambdaYES-R, pTRP
Host range: Escherichia coli
Features (with orientation and position when available):
other: left arm (19.6 kb), ->
other: lox, ->
promoter for expression: GAL1, ->
MCS: EcoRI...SacI, ->
transcription terminator: CYC1, ->
marker(s): TRP1, ->
replicon: 2 micron
marker(s): ampR, ->
replicon: pMB1
replicon: oriT
other: right arm (15.2 kb, with cI857)
Cross references:
Insert size range: 0.7 to 7 kb
Inserts ends: XhoI adaptors
Insert site: XhoI
Number of independent recombinants: 1 x 10^8
Non-recombinants (%): 5
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen bacteria-free lysate (volume: 0.4 mL)
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae